Human UNC13B Antibody

Catalog #: MAB6188 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB6188
MAB6188-SP

All UNC13B Antibodies

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human UNC13B Antibody Summary

Species Reactivity

Human

Specificity

Detects human UNC13B in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) UNC13A and rhUNC13C is observed.

Source

Monoclonal Mouse IgG_2B Clone # 584102

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. Coli-derived recombinant human UNC13B
Asp418-Ser1591
Accession # O14795

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Immunohistochemistry

8-25 µg/mL

Immersion-fixed paraffin-embedded sections of human kidney and human kidney cancer tissue

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: UNC13B

UNC13B is a 170 kDa member of the unc13 family. It is 1591 amino acids (aa) in length and contains three C2 domains, one MHD1 domain, one MHD2 domain, and one phorbol-ester/DAG-type zinc finger. Human UNC13B is 95% and 93% aa identical to mouse and rat UNC13B, respectively. UNC13B is expressed in brain and kidney cortical epithelial and mesangial cells. UNC13B plays a role in vesicle maturation during exocytosis as a target of the diacylglycerol second messenger pathway. It is involved in neurotransmitter release by acting in synaptic vesicle priming prior to vesicle fusion and participates in the activity-depending refilling of readily releasable vesicle pool (RRP). In addition, UNC13B may be involved in mediating some of the acute and chronic changes in mesangial cells produced by exposure to hyperglycemia

Long Name

UNC-13 Homolog B

Entrez Gene IDs

10497 (Human)

Alternate Names

hmunc13; homolog of rat Munc13 (diacylglycerol-binding); MGC133279; MGC133280; MUNC13; munc13-2; MUNC13B; protein unc-13 homolog B; unc-13 homolog B (C. elegans); UNC13B; Unc13h2; unc-13-like (C. elegans); UNC13Unc13h2