Human USAG1 Antibody

Catalog #: AF7034 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF7034
AF7034-SP

Detection of Human USAG1 by Western Blot.

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Human USAG1 Antibody Summary

Species Reactivity

Human

Specificity

Detects human USAG1 in direct ELISAs and Western blots.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human USAG1
Phe24-Ser206
Accession # Q6X4U4

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human USAG1 by Western Blot. Western blot shows lysates of human kidney (medulla) tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human USAG1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7034) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for USAG1 at approximately 32 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.2 mg/mL.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: USAG1

USAG1 (also Ectodin and WISE; Wnt modulator in surface ectoderm) is a secreted, monomeric 26-32 kDa member of the sclerostin family of proteins. It exhibits restricted expression, being found in renal distal tubule cells (including macula densa), ameloblasts of teeth (for enamel formation), and cells of the dermal papilla. USAG1 binds BMP-2,-4,-6,and -7 with high affinity. This sequesters BMPs, making them unavailable for BMP receptor binding. It also binds LRP6, which, while weakly activating the LRP6 pathway, simultaneously blocks Wnt binding with strong Wnt-mediated signaling. Mature human USAG1 is 183 amino acids (aa) in length. It contains one CTCK domain (aa 75-170) plus two N-linked glycosylation sites. Mature human USAG1 (aa 24-206) shares 97% aa identity with mature mouse USAG1.

Long Name

Sclerostin Domain Containing Protein 1/Uterine Sensitization-associated Gene 1 Protein

Entrez Gene IDs

25928 (Human); 66042 (Mouse); 266803 (Rat)

Alternate Names

CDA019; cystine-knot containing secreted protein; DKFZp564D206; Ectodermal BMP inhibitor; Ectodin; sclerostin domain containing 1; sclerostin domain-containing protein 1; SOSTDC1; USAG1; USAG-1; USAG1uterine sensitization-associated protein-1; Uterine sensitization-associated gene 1 protein; WISE