Human VAMP-7 Antibody

Detection of Human VAMP‑7 by Western Blot. Western blot shows lysates of A172 human glioblastoma cell line and K562 human chronic myelogenous leukemia cell line. PVDF Membrane was probed with 1 µg/mL of Mouse Anti-Human VAMP-7 Monoclonal Antibody (Catalog # MAB6117) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for VAMP-7 at approximately 25 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Detection of Human VAMP-7 by Western Blot Amphisome/lysosome fusion is not required for autophagy-mediated exosomal secretion of ANXA2. (a) Cells with VAMP7 knockdown and control knockdown were transfected with a GFP-LC3 plasmid and treated with or without 500 U/ml IFN-gamma for 24 h. Cells were then fixed, permeabilized, and stained for ANXA2 (red) and LAMP1 (blue). The colocalization of ANXA2, GFP-LC3 and LAMP1 was observed by confocal microscopy. Scale bar: 10 μm. The arrow and dotted inset mark an autolysosome. (b) Line tracing analysis of fluorescence signal from image in (a) of VAMP7 knockdown and control knockdown cells after IFN-gamma stimulation is shown. (c) VAMP7 knockdown efficiency was detected by western blotting. Control and VAMP7-silenced cells were treated with or without 500 U/ml IFN-gamma for 48 h. The exosome pellets were collected. ANXA2, alpha -tubulin, Tsg101 and calnexin from exosome pellets and total cell lysates were detected by western blotting. kD, molecular weight as kDa. (d) A549 cells were incubated with 500 U/ml IFN-gamma in the presence or absence of 5 nM bafilomycin A1 for 48 h. ANXA2 and alpha -tubulin from cultured supernatant and total cell lysate were analyzed by western blotting. kD, molecular weight as kDa. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/28720835), licensed under a CC-BY license. Not internally tested by R&D Systems.