Human Vang-like Protein 1/VANGL1 Antibody

Catalog #: MAB5476 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB5476
MAB5476-SP

Vang-like Protein 1/VANGL1 in HeLa Human Cell Line.

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All Vang-like Protein 1/VANGL1 Antibodies

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Vang-like Protein 1/VANGL1 Antibody Summary

Species Reactivity

Human

Specificity

Detects human Vang-like Protein 1/VANGL1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, slight cross-reactivity with recombinant human VANGL2 is observed.

Source

Monoclonal Mouse IgG_2B Clone # 497707

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human Vang-like Protein 1/VANGL1
Glu242-Val524
Accession # Q8TAA9

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

Recombinant Human Vang-like Protein 1/VANGL1

Immunocytochemistry

8-25 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry

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Vang-like Protein 1/VANGL1 in HeLa Human Cell Line. Vang-like Protein 1/VANGL1 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Human Vang-like Protein 1/VANGL1 Monoclonal Antibody (Catalog # MAB5476) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counter-stained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Vang-like Protein 1/VANGL1

Human Van Gogh-like protein 1 (VANGL1; also Vang-like Protein 1, strabismus 2, loop tail protein 2 homolog, LPP2) is a 60 kDa (predicted) member of the Vang family of proteins. VANGL1 is 524 amino acids (aa) in length and contains four transmembrane regions and the C-terminal Ser/Thr-X-Val motif, but lacks a signal sequence. There are two isoforms for VANGL1. In isoform 2, residues 67-68 found in isoform 1 are deleted. Human VANGL1 shares 95% aa sequence identity with mouse VANGL1. The glycoprotein is ubiquitously expressed in normal tissues and is upregulated in many cancer cell lines. VANGL1 is predicted to be a tumor suppressor gene. Mutations in VANGL1 are associated with neural tube defects.

Entrez Gene IDs

81839 (Human); 229658 (Mouse); 690366 (Rat)

Alternate Names

KAI1 C-terminal interacting tetraspanin; Loop-tail protein 2 homolog; LPP2; MGC5338; STB2; STB2KITENIN; STBM2; Strabismus 2; Van Gogh-like protein 1; vang (van gogh, Drosophila)-like 1, vang, van gogh-like 1 (Drosophila); Vang like Protein 1; VANGL1; vang-like 1 (van gogh, Drosophila); Vang-like Protein 1