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Human Vasohibin Isoform 1 Antibody

Catalog #: AF3319
1 Citation Datasheet / COA / SDS
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AF3319
AF3319-SP
Product Details
Citations (1)
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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Vasohibin Isoform 1 Antibody Summary

Species Reactivity
Human
Specificity
Detects human Vasohibin Isoform 1 in direct ELISAs and Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Vasohibin Isoform 1
Pro2-Val365
Accession # Q7L8A9
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human Vasohibin Isoform 1

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Vasohibin

Vasohibin is a secreted endothelium-derived negative feedback regulator of angiogenesis whose expression in endothelial cells is induced by proangiogenic growth factors such as VEGF and FGF-2. It is synthesized as a 365 amino acid residue protein that lacks a canonical signal peptide. As a result of proteolytic cleavage, multiple distinct molecular weight forms can be detected when Vasohibin is expressed on endothelial cells. Human and mouse Vasohibin share 93% amino acid sequence identity. Two splice variants of human Vasohibin have been reported.

Entrez Gene IDs
22846 (Human); 238328 (Mouse)
Alternate Names
KIAA1036; KIAA1036vasohibin-1; VASH; VASH1; vasohibin 1; Vasohibin

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Citation for Human Vasohibin Isoform 1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Alternative splicing of vasohibin-1 generates an inhibitor of endothelial cell proliferation, migration, and capillary tube formation.
    Authors: Kern J, Bauer M, Rychli K, Wojta J, Ritsch A, Gastl G, Gunsilius E, Untergasser G
    Arterioscler. Thromb. Vasc. Biol., 2008-01-10;28(3):478-84.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot

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