Human Vav-1 Antibody

Catalog #: AF3786
1 Citation Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF3786
AF3786-SP

Detection of Human Vav‑1 by Western Blot.

1 Image

All Vav-1 Antibodies

Product Details

Citations (1)

Supplemental Products

Reviews

Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Vav-1 Antibody Summary

Species Reactivity

Human

Specificity

Detects human Vav-1 in Western blots.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human Vav-1
Trp25-Leu228
Accession # P15498

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

View Larger

Detection of Human Vav‑1 by Western Blot. Western blot shows lysates of Jurkat human acute T cell leukemia cell line and Raji human Burkitt's lymphoma cell line. PVDF membrane was probed with 1 µg/mL of Human Vav-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3786) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Vav-1 at approximately 110 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Vav-1

The Vav-1 proto-oncogene is a guanine nucleotide exchange factor (GEF) for the low molecular weight GTPases of the Rho/Rac family. Vav-1 promotes intracellular signaling by its multiple protein binding motifs. It is a cytosolic protein primarily expressed in hematopoietic cells and plays an essential role in the proliferation and activation of T and B cells.

Entrez Gene IDs

7409 (Human); 22324 (Mouse); 25156 (Rat)

Alternate Names

oncogene vav; proto-oncogene vav; vav 1 guanine nucleotide exchange factor; vav proto-oncogene; Vav1; Vav-1; vav-T; VAVvav 1 oncogene

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citation for Human Vav-1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1