Human VIP Antibody

Name: Human VIP Antibody
Brand: R&D Systems
SKU: MAB6079
Rating: 5 (1 reviews)

Catalog #: MAB6079 1 Review Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB6079
MAB6079-SP

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Human VIP Antibody Summary

Species Reactivity

Human

Specificity

Detects recombinant human VIP in direct ELISAs. It does not detect the C-terminal region (aa 145-152).

Source

Monoclonal Mouse IgG₁ Clone # 576721

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

KLH-coupled human VIP synthetic peptides HSDAVFTDNYTR and KYLNSILN

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Immunohistochemistry

8-25 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry

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VIP in Human Stomach. VIP was detected in immersion fixed paraffin-embedded sections of human stomach array using Mouse Anti-Human VIP Monoclonal Antibody (Catalog # MAB6079) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: VIP

Vasoactive intestinal peptide (VIP) is a 28 amino acid (aa) peptide that exerts wide-ranging effects in embryonic and adult tissues through interactions with the 7‑transmembrane spanning receptors VIP R1 and VIP R2. The VIP proprotein is proteolytically processed to release bioactive VIP as well as the bioactive peptides PHV and PHM. VIP regulates appetite and feeding behavior and stimulates insulin and glucagon secretion in pancreatic islet cells. It also regulates astrocyte function, multiple aspects of cardiac function, and TLR function in innate immune responses. Mature VIP shares 100% aa sequence identity with mouse and rat VIP.

Long Name

Vasoactive Intestinal Peptide

Entrez Gene IDs

7432 (Human)

Alternate Names

MGC13587; PHM27; vasoactive intestinal peptide; VIP peptides; VIP

Product Datasheets

Product Datasheet

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FAQs

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Reviews for Human VIP Antibody

Average Rating: 5 (Based on 1 Review)

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Human VIP Antibody

By Anonymous on 02/21/2022

Application: IHC Sample Tested: Stomach tissue Species: Human

Immunohistochemistry Human VIP Antibody MAB6079