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Human Vitamin D BP Antibody

Catalog #: MAB3778
1 Citation Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB3778
MAB3778-SP
Product Details
Citations (1)
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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Vitamin D BP Antibody Summary

Species Reactivity
Human
Specificity
Detects human plasma-derived Vitamin D BP in direct ELISAs and Western blots.
Source
Monoclonal Mouse IgG2B Clone # 359501
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Human plasma-derived Vitamin D BP
Accession # P02774
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS and NaCl with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Human plasma-derived Vitamin D BP

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Vitamin D BP

Vitamin D BP (VitD BP), also called VDB, DBP, Group Specific Component (Gc) or macrophage-activating factor (DBP- or Gc-MAF), is a member of the albumin family. These plasma/body fluid proteins have a conserved pattern of cysteine motifs that form binding domains. VitD BP is predominantly produced by hepatic parenchymal cells. It transports VitD, activates macrophages and osteoclasts, and enhances C5a chemotactic activity. Over 80 variant alleles have been identified, some of which correlate with osteoporosis or prostate cancer. Human and mouse VitD BP share 78% aa identity.

Long Name
Vitamin D Binding Protein
Entrez Gene IDs
2638 (Human); 14473 (Mouse); 24384 (Rat)
Alternate Names
DBP; DBP/GC; DBP-MAF; DBPVDBG; EC 6.3.1.5; Gc; gc-globulin; Gc-MAF; GRD3; group-specific component (vitamin D binding protein); Group-specific component; hDBP; VDB; VDBG; VDBP; Vitamin D BP; vitamin D-binding alpha-globulin; vitamin D-binding protein

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Citation for Human Vitamin D BP Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Unique Transcriptional Programs Identify Subtypes of AKI
    Authors: Katherine Xu, Paul Rosenstiel, Neal Paragas, Christian Hinze, Xiaobo Gao, Tian Huai Shen et al.
    Journal of the American Society of Nephrology

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