Human VSIG10 Antibody

Catalog #: MAB9969 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB9969-100
MAB9969-SP

VSIG10 in RT-4 and MOLT-4 Human Cell Lines.

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Human VSIG10 Antibody Summary

Species Reactivity

Human

Specificity

Detects human VSIG10 in direct ELISAs.

Source

Monoclonal Mouse IgG₁ Clone # 988622

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Human embryonic kidney cell, HEK293-derived human VSIG10
Met1-Gly413
Accession # Q8N0Z9

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Immunocytochemistry

8-25 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry

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VSIG10 in RT-4 and MOLT-4 Human Cell Lines. VSIG10 was detected in immersion fixed RT-4 human urinary bladder transitional cell papilloma cell line (left panel) and MOLT-4 human acute lymphoblastic leukemia cell line (negative control; right panel) using Mouse Anti-Human VSIG10 Monoclonal Antibody (Catalog # MAB9969) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: VSIG10

VSIG10 (V-set and immunoglobulin domain-containing protein 10) is a single-pass type I membrane protein of the Ig superfamily. Human VSIG10 cDNA encodes a 540 amino acid (aa) precursor that contains a 30 aa signal sequence, a 383 aa extracellular domain (ECD), a 21 aa transmembrane sequence, and a 106 aa cytoplasmic domain. Human VSIG10 ECD contains four Ig-like C2-type domains and shares 95%, 69% and 71% aa identity with chimpanzee, mouse and rat VSIG10, respectively. VSIG10 is structurally related to the B7 family of immune regulatory proteins.

Long Name

V-Set and Immunoglobulin Domain Containing 10

Entrez Gene IDs

54621 (Human); 231668 (Mouse); 304529 (Rat)

Alternate Names

FLJ20674; V-set and immunoglobulin domain containing 10; V-set and immunoglobulin domain-containing protein 10; VSIG10

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

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