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Human VSIG8 Antibody

Catalog #: MAB9418 Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB9418-100
MAB9418-SP
Detection of VSIG8 in HEK293 Human Cell Line Transfected with Human VSIG8 and eGFP by Flow Cytometry.
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Human VSIG8 Antibody Summary

Species Reactivity
Human
Specificity
Detects human VSIG8 in direct ELISAs. Stains human VSIG8 transfectants but not irrelevant transfectants in flow cytometry.
Source
Monoclonal Mouse IgG1 Clone # 961823
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Human embryonic kidney cell line HEK293-derived transfected with human VSIG8
Met1-Gly263
Accession # Q5VU13
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
ELISA

This antibody functions as an ELISA capture antibody when paired with Mouse Anti-Human VSIG8 Monoclonal Antibody (Catalog # MAB94181).

This product is intended for assay development on various assay platforms requiring antibody pairs. We recommend the Human VSIG8 DuoSet ELISA Kit (Catalog # DY9200-05) for convenient development of a sandwich ELISA.

 
Flow Cytometry
0.25 µg/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of VSIG8 antibody in HEK293 Human Cell Line Transfected with Human VSIG8 and eGFP antibody by Flow Cytometry. View Larger

Detection of VSIG8 in HEK293 Human Cell Line Transfected with Human VSIG8 and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with either (A) human VSIG8 or (B) irrelevant transfectants and eGFP was stained with Mouse Anti-Human VSIG8 Monoclonal Antibody (Catalog # MAB9418) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). Quadrant markers were set based on control antibody staining (Catalog # MAB002). View our protocol for Staining Membrane-associated Proteins.

ELISA Human VSIG8 Antibody in ELISA Standard Curve. View Larger

Human VSIG8 ELISA Standard Curve. Recombinant Human VSIG8 protein was serially diluted 2-fold and captured by Mouse Anti-Human VSIG8 Monoclonal Antibody (Catalog # MAB9418) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human VSIG8 Monoclonal Antibody (Catalog # MAB94181) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: VSIG8

VSIG8 (V-set and immunoglobulin domain containing 8), also known as C1orf204, is an approximately 45 kDa type I transmembrane protein of the B7 family within the Ig superfamily. Mature human VSIG8 consists of a 242 amino acid (aa) extracellular domain (ECD) containing two V-type Ig-like domains, a 21 aa transmembrane domain, and a 130 aa cytoplasmic domain. Within the ECD, human VSIG8 shares 88% and 89% aa identity with mouse and rat VSIG8, respectively. Alternative splicing generates a long isoform of human VSIG8 with a substitution in the cytoplasmic juxtamembrane region and a 124 aa extension at the C-terminus. VSIG8 was identified from proteomic analysis of human hair shafts (1, 2). It is expressed in the hair follicle and shaft, superficial layers of the nail matrix, and superficial layers of oral epithelium (3). R&D Systems in-house testing indicates that VSIG8 inhibits the production of IL-2 by activated T cells.

References
  1. Rice, R.H. et al. (2010) J. Proteome Res. 9:6752.
  2. Lee, Y.J. et al. (2006) Mol. Cell. Proteomics 5:789.
  3. Rice, R.H. et al. (2011) J. Invest. Dermatol. 131:1936.
Long Name
V-Set and Immunoglobulin Domain Containing 8
Entrez Gene IDs
391123 (Human); 240916 (Mouse); 289236 (Rat); 102123183 (Cynomolgus Monkey)
Alternate Names
V-set and immunoglobulin domain containing 8; VSIG8

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