Human VSTM2A Antibody

Catalog #: MAB100371 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB100371-100
MAB100371-SP

Detection of VSTM2A in HEK293 Human Cell Line transfected with Human VSTM2A and eGFP by Flow Cytometry

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All VSTM2A Antibodies

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human VSTM2A Antibody Summary

Species Reactivity

Human

Specificity

Detects human VSTM2A in direct ELISAs.

Source

Monoclonal Mouse IgG_2B Clone # 1024735

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Human embryonic kidney cell HEK293-derived human VSTM2A protein
Ser25-Phe244
Accession # Q8TAG5-2

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

ELISA

This antibody functions as an ELISA detection antibody when paired with Mouse Anti-Human VSTM2A Monoclonal Antibody (Catalog # MAB10037).

This product is intended for assay development on various assay platforms requiring antibody pairs.

Flow Cytometry

0.25 µg/10⁶ cells

HEK293 Human Cell Line transfected with Human VSTM2A and eGFP

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of VSTM2A in HEK293 Human Cell Line transfected with Human VSTM2A and eGFP by Flow Cytometry HEK293 human embryonic kidney cell line transfected with either (A) human VSTM2A or (B) irrelevant protein, and eGFP, was stained with Mouse anti-human VSTM2A monoclonal antibody (Catalog # MAB100371) followed by Allophycocyanin-conjugated anti-Mouse IgG Secondary Antibody (F0101B). Quadrant markers were set based on control antibody staining (MAB10041, data not shown). Staining was performed using our Staining Membrane-Associated Proteins protocol.

ELISA

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Human VSTM2A ELISA Standard Curve. Recombinant Human VSTM2A protein was serially diluted 2-fold and captured by Mouse Anti-Human VSTM2A Monoclonal Antibody (MAB10037) coated on a Clear Polystyrene Microplate (DY990). Mouse Anti-Human VSTM2A Monoclonal Antibody (Catalog # MAB100371) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (DY998) followed by Substrate Solution (DY999) and stopping the enzymatic reaction with Stop Solution (DY994).

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: VSTM2A

V-set and transmembrane domain-containing protein 2A (VSTM2A) is a secreted glycoprotein that is expressed by committed preadipocytes. N-linked glycosylation is crucial for its secretion, but not for preadipocyte cell differentiation activity. It is expressed during adipocyte development and its over-expression promotes adipogenesis (1). VSTM2A is highly expressed in the brain and Vstm2a was identified as an enigmatic gene that is highly produced in mouse brain (1, 2). A positive association has been observed between Vstm2a and Pparg2. VSTM2A plays a role in the regulation of the early stage of white and brown preadipocyte cell differentiation. It promotes adipogenic commitment of preadipocytes by increasing gene expression of the transcription factor PPARG in a BMP4-dependent signaling pathway (1, 3). In humans, two isoforms (1 and 2) exist due to alternative splicing. Human VSTM2A is synthesized either as a 236 amino acid (aa) (Isoform 1) or a 244 aa (Isoform 2) precursor that contains a 24 aa signal sequence followed by the VSTM2A domain. Human Isoform 2 VSTM2A shares 78% aa sequence identity with mouse and rat VSTM2A.

References

Secco, B. et al. (2017) Cell Rep. 18:93.
Pandey, A.K. et al. (2014) PloS One. 9:e88889.
Berry, D.C. et al. (2013) Development. 140:3939.

Long Name

V-Set And Transmembrane Domain Containing 2A

Entrez Gene IDs

222008 (Human); 211739 (Mouse); 689106 (Rat)

Alternate Names

MGC33530; V-set and transmembrane domain containing 2; V-set and transmembrane domain containing 2A; V-set and transmembrane domain-containing protein 2A; VSTM2; VSTM2A