Human Wnt-10b Antibody Summary
Asn29-Lys389
Accession # O00744
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human Wnt‑10b by Western Blot. Western blot shows lysates of SW480 human colorectal adenocarcinoma cell line and MDA-MB-468 human breast cancer cell line. PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human Wnt-10b Monoclonal Antibody (Catalog # MAB7196) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Wnt-10b at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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Detection of Human Wnt‑10b by Simple WesternTM. Simple Western lane view shows lysate of SW480 human colorectal adenocarcinoma cell line, loaded at 0.5 mg/mL. A specific band was detected for Wnt‑10b at approximately 58 kDa (as indicated) using 5 µg/mL of Mouse Anti-Human Wnt‑10b Monoclonal Antibody (Catalog # MAB7196). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system. Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Wnt-10b
Wnt-10b (also known as Wnt-12) is a 42‑44 kDa member of the Wnt family of secreted, highly conserved, cysteine-rich glycoproteins that play important roles in vertebrate pattern formation, cell fate decision, axon guidance, and tumor formation (1‑3). Human Wnt-10b cDNA encodes a 389 amino acid (aa) precursor that contains a 28 aa signal sequence plus a 361 aa mature protein that contains two glycosylation sites, three potential phosphorylation sites, and a potential palmitoylation site (3, 4). Human Wnt-10b shares 97‑99% aa identity with mouse, rat, equine, porcine, and canine Wnt-10b. Wnt-10b plays a critical role in maintaining mesenchymal stem cells and determining whether they differentiate to adipocytes or osteoblasts (5‑7). Mouse Wnt-10b deletion produces age‑dependent loss of bone mass due to defective production of osteoblasts, while transgenic over‑expression increases postnatal osteoblast differentiation and inhibits adipocyte differentiation (5‑7). Ectopic expression of Wnt-10b in an obesity and diabetes‑prone background, such as the ob/ob mouse, inhibits obesity (8). In mouse skeletal muscle, Wnt-10b is expressed inversely with SREBP1c and increases insulin sensitivity (9). In humans, a missense polymorphism is responsible for a malformation of hands and feet, while a C256Y inactivating mutation is associated with severe early-onset obesity (10, 11). Wnt-10b is mainly produced by stem cells and pre-osteoblasts, but also by adult bone marrow CD8+ T lymphocytes stimulated with parathyroid hormone (12). In some hepatocellular carcinomas, Wnt-10b can inhibit cancer cell growth, but in others, it can act synergistically with FGFs to stimulate cell growth (13). Several Wnts, including Wnt-10b, are expressed in both normal and/or malignant colon tissues (14).
- Hardiman, G. et al. (1997) Cytogenet. Cell Genet. 77:278.
- Katoh, M. (2008) Curr. Drug Targets 9:565.
- Hausmann, G. et al. (2007) Nat. Rev. Mol. Cell Biol. 8:331.
- Swiss Prot Accession # O00744.
- Stevens, J.R. et al. (2010) J. Bone Miner. Res. 25:2138.
- Bennett, C.N. et al. (2007) J. Bone Miner. Res. 22:1924.
- Bennett, C.N. et al. (2005) Proc. Natl. Acad. Sci. USA 102:3324.
- Wright, W.S. et al. (2007) Diabetes 56:295.
- Abiola, M. et al. (2009) PLoS ONE 4:e8509.
- Ugur, S.A. and A. Tolun (2008) Hum. Mol. Genet. 17:2644.
- Christodoulides, C. et al. (2006) Diabetologia 49:678.
- Terauchi, M. et al. (2009) Cell Metab. 10:229.
- Yoshikawa, H. et al. (2007) Mol. Biol. Cell 18:4292.
- Holcombe, R.F. et al. (2002) J. Clin. Pathol: Mol. Pathol. 55:220.
Product Datasheets
Citation for Human Wnt-10b Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
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T cells detect intracellular DNA but fail to induce type I IFN responses: implications for restriction of HIV replication.
Authors: Berg R, Rahbek S, Kofod-Olsen E, Holm C, Melchjorsen J, Jensen D, Hansen A, Jorgensen L, Ostergaard L, Tolstrup M, Larsen C, Paludan S, Jakobsen M, Mogensen T
PLoS ONE, 2014-01-03;9(1):e84513.
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