Human Wnt-7b Antibody

Discontinued Product

MAB3460 has been discontinued.
View all Wnt-7b products.
Detection of Human Wnt‑7b by Western Blot.
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Product Details
Citations (1)
FAQs
Reviews (1)

Human Wnt-7b Antibody Summary

Species Reactivity
Human
Specificity
Detects human Wnt-7b in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross‑reactivity with recombinant human Wnt-1, -2, -7a, -9a, recombinant mouse Wnt-2b, -3a, -4, -5a, -5b, -8a, -8b, -9b, -10a, -10b, -11, or -16 is observed. Was not tested for cross‑reactivity with mouse Wnt-7b.
Source
Monoclonal Mouse IgG2A Clone # 355104
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human Wnt‑7b
Leu32-Glu72 & Thr216-Ala283
Accession # NP_478679
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.

Applications

Recommended Concentration
Sample
Western Blot
5 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human Wnt‑7b antibody by Western Blot. View Larger

Detection of Human Wnt‑7b by Western Blot. Western blot shows lysates of MCF-7 human breast cancer cell line and RL95-2 human endometrial carcinoma cell line. PVDF membrane was probed with 5 µg/mL Mouse Anti-Human Wnt-7b Monoclonal Antibody (Catalog # MAB3460) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). For additional reference, recombinant human Wnt-7b (aa 32-72, 20 ng) was included. A specific band for Wnt-7b was detected at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 6.

Reconstitution Calculator

Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Wnt-7b

Human Wnt-7b is a 46 kDa, secreted, glycosylated member of the Wnt family of developmental proteins. It is considered a class 3 Wnt based on its inability to transform C57MG cells. Human Wnt-7b is synthesized as a 349 aa precursor that contains a 318 aa mature region. The mature region contains 24 cysteines and three potential N-linked glycosylation sites. Mature human Wnt-7b is 99% identical to mature mouse and rat Wnt-7b and 80% identical to Wnt-7a. Wnt-7b is produced by mammary duct epithelium.

Long Name
Wingless-type MMTV Integration Site Family, Member 7B
Entrez Gene IDs
7477 (Human); 22422 (Mouse); 315196 (Rat)
Alternate Names
protein Wnt-7b; wingless-type MMTV integration site family, member 7B; Wnt7b; Wnt-7b

Product Datasheets

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Citation for Human Wnt-7b Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Wnt signaling mediates experience-related regulation of synapse numbers and mossy fiber connectivities in the adult hippocampus.
    Authors: Gogolla N, Galimberti I, Deguchi Y, Caroni P
    Neuron, 2009-05-28;62(4):510-25.
    Species: Mouse
    Sample Types: Tissue Homogenates, Whole Tissue
    Applications: IHC-Fr, Western Blot

FAQs

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Reviews for Human Wnt-7b Antibody

Average Rating: 3 (Based on 1 Review)

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Human Wnt-7b Antibody
By Anonymous on 02/21/2019
Application: MiAr Sample Tested: EDTA Plasma Species: Human