Human Wnt-7b Antibody

Name: Human Wnt-7b Antibody
Brand: R&D Systems
SKU: MAB3460
Rating: 3 (1 reviews)

Catalog #: MAB3460
1 Citation 1 Review Datasheet / COA / SDS

Discontinued Product

MAB3460 has been discontinued.

View all Wnt-7b products.

Detection of Human Wnt‑7b by Western Blot.

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All Wnt-7b Antibodies

Product Details

Citations (1)

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Reviews (1)

Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Wnt-7b Antibody Summary

Species Reactivity

Human

Specificity

Detects human Wnt-7b in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross‑reactivity with recombinant human Wnt-1, -2, -7a, -9a, recombinant mouse Wnt-2b, -3a, -4, -5a, -5b, -8a, -8b, -9b, -10a, -10b, -11, or -16 is observed. Was not tested for cross‑reactivity with mouse Wnt-7b.

Source

Monoclonal Mouse IgG_2A Clone # 355104

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human Wnt‑7b
Leu32-Glu72 & Thr216-Ala283
Accession # NP_478679

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.

Applications

Recommended Concentration

Sample

Western Blot

5 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human Wnt‑7b by Western Blot. Western blot shows lysates of MCF-7 human breast cancer cell line and RL95-2 human endometrial carcinoma cell line. PVDF membrane was probed with 5 µg/mL Mouse Anti-Human Wnt-7b Monoclonal Antibody (Catalog # MAB3460) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). For additional reference, recombinant human Wnt-7b (aa 32-72, 20 ng) was included. A specific band for Wnt-7b was detected at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 6.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Wnt-7b

Human Wnt-7b is a 46 kDa, secreted, glycosylated member of the Wnt family of developmental proteins. It is considered a class 3 Wnt based on its inability to transform C57MG cells. Human Wnt-7b is synthesized as a 349 aa precursor that contains a 318 aa mature region. The mature region contains 24 cysteines and three potential N-linked glycosylation sites. Mature human Wnt-7b is 99% identical to mature mouse and rat Wnt-7b and 80% identical to Wnt-7a. Wnt-7b is produced by mammary duct epithelium.

Long Name

Wingless-type MMTV Integration Site Family, Member 7B

Entrez Gene IDs

7477 (Human); 22422 (Mouse); 315196 (Rat)

Alternate Names

protein Wnt-7b; wingless-type MMTV integration site family, member 7B; Wnt7b; Wnt-7b

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citation for Human Wnt-7b Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1