Human Wnt-7b Antibody
Human Wnt-7b Antibody Summary
Leu32-Glu72 & Thr216-Ala283
Accession # NP_478679
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data

Detection of Human Wnt‑7b by Western Blot. Western blot shows lysates of MCF-7 human breast cancer cell line and RL95-2 human endometrial carcinoma cell line. PVDF membrane was probed with 5 µg/mL Mouse Anti-Human Wnt-7b Monoclonal Antibody (Catalog # MAB3460) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). For additional reference, recombinant human Wnt-7b (aa 32-72, 20 ng) was included. A specific band for Wnt-7b was detected at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 6.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Wnt-7b
Human Wnt-7b is a 46 kDa, secreted, glycosylated member of the Wnt family of developmental proteins. It is considered a class 3 Wnt based on its inability to transform C57MG cells. Human Wnt-7b is synthesized as a 349 aa precursor that contains a 318 aa mature region. The mature region contains 24 cysteines and three potential N-linked glycosylation sites. Mature human Wnt-7b is 99% identical to mature mouse and rat Wnt-7b and 80% identical to Wnt-7a. Wnt-7b is produced by mammary duct epithelium.
Product Datasheets
Citation for Human Wnt-7b Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
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Wnt signaling mediates experience-related regulation of synapse numbers and mossy fiber connectivities in the adult hippocampus.
Authors: Gogolla N, Galimberti I, Deguchi Y, Caroni P
Neuron, 2009-05-28;62(4):510-25.
Species: Mouse
Sample Types: Tissue Homogenates, Whole Tissue
Applications: IHC-Fr, Western Blot
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