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Human XCL1/Lymphotactin Antibody

Catalog #: AF695
2 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF695
AF695-SP
Chemotaxis Induced by XCL1/Lymphotactin and Neutralization by Human XCL1/Lymphotactin Antibody.
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Citations (2)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human XCL1/Lymphotactin Antibody Summary

Species Reactivity
Human
Specificity
Ddetects human XCL1/Lymphotactin in direct ELISAs and Western blots. 
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human XCL1/Lymphotactin
Val22-Gly114
Accession # P47992
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human XCL1/Lymphotactin (Catalog # 695-LT)
Neutralization
Measured by its ability to neutralize XCL1/Lymphotactin-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human XCR1. The Neutralization Dose (ND50) is typically 3.00-30.0 µg/mL in the presence of 0.5 µg/mL Recombinant Human XCL1/Lymphotactin.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization Chemotaxis Induced by XCL1/Lymphotactin and Neutralization by Human XCL1/Lymphotactin Antibody. View Larger

Chemotaxis Induced by XCL1/Lymphotactin and Neutralization by Human XCL1/Lymphotactin Antibody. Recombinant Human XCL1/ Lymphotactin (695-LT) chemoattracts the BaF3 mouse pro-B cell line transfected with human XCR1 in a dose dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (AR002). Chemotaxis elicited by Recombinant Human XCL1/ Lymphotactin (0.5 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human XCL1/ Lymphotactin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF695). The ND50 is typically 3.00-30.0 µg/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: XCL1/Lymphotactin

Human lymphotactin (Lptn)/XCL1 (also named human SCM-1 alpha and ATAC) and its mouse homologue belong to the C or gamma  subfamily of chemokines. The C chemokines lack two (the 1st and 3rd ) of the four invariant cysteine residues normally found in the CC and CXC chemokines and have an extended carboxy terminus. Human lymphotactin encodes a 114 amino acid residue precursor protein with a 21 amino acid residue predicted signal peptide. The expression of lymphotactin is abundant in some activated T cells such as activated CD8+ T cells and other class I MHC restricted T cells. Lptn expression is absent in CD4+ T cells. Human and mouse Lptn share approximately 60% amino acid sequence homology. The gene for lymphotactin has been mapped to chromosome 1 in both human and mouse. Recombinant human lymphotactin has been shown to have chemotactic activity for lymphocytes and NK cells. The orphan receptor GPR5 has been reported to be the specific receptor for Lptn.

References
  1. Kennedy, J. et al. (1995) J. Immunol. 155:203.
  2. Zlotnik, A. et al. (1999) Critical Reviews in Immunol. 19:1.
Long Name
Chemokine [C Motif] Ligand 1
Entrez Gene IDs
6375 (Human); 16963 (Mouse)
Alternate Names
ATAC; ATACSmall-inducible cytokine C1; C motif chemokine 1; chemokine (C motif) ligand 1; Cytokine SCM-1; LPTNSCM-1-alpha; LTNXC chemokine ligand 1; Lymphotactin; lymphotaxin; SCM-1 alpha; SCM-1; SCM1A; SCM-1a; SCYC1SCM1; single cysteine motif 1a; small inducible cytokine subfamily C, member 1 (lymphotactin); XCL1

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Citations for Human XCL1/Lymphotactin Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. The CD8-derived chemokine XCL1/lymphotactin is a conformation-dependent, broad-spectrum inhibitor of HIV-1.
    Authors: Guzzo C, Fox J, Lin Y, Miao H, Cimbro R, Volkman B, Fauci A, Lusso P
    PLoS Pathog, 2013-12-26;9(12):e1003852.
    Species: Human, Virus - HIV-1
    Sample Types: Antibody, Recombinant Protein
    Applications: Capture Assay, Co-Immunoprecipitation
  2. Comprehensive assessment of chemokine expression profiles by flow cytometry.
    Authors: Eberlein J, Nguyen TT, Victorino F, Golden-Mason L, Rosen HR, Homann D
    J. Clin. Invest., 2010-02-08;120(3):907-23.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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