Human XCL1/Lymphotactin Biotinylated Antibody

Catalog #: BAF695
1 Citation Datasheet / COA / SDS

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BAF695

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Human XCL1/Lymphotactin Biotinylated Antibody Summary

Species Reactivity

Human

Specificity

Detects human XCL1 in ELISAs and Western blots. In sandwich immunoassays, less than 0.15% cross-reactivity with recombinant mouse XCL1, recombinant human (rh) Eotaxin, and rhEotaxin-2 is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human XCL1/Lymphotactin (R&D Systems, Catalog # 695-LT)
Val22-Gly114
Accession # P47992

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Label

Biotin

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Human XCL1/Lymphotactin (Catalog # 695-LT)

Human XCL1/Lymphotactin Sandwich Immunoassay

Recommended Concentration

Reagent

ELISA Detection (Matched Antibody Pair)

0.1-0.4 µg/mL

Use in combination with:

Capture Reagent: Human XCL1/Lymphotactin Antibody (Catalog # MAB6951)

Standard: Recombinant Human XCL1/Lymphotactin Protein (Catalog # 695-LT)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: XCL1/Lymphotactin

Human lymphotactin (Lptn)/XCL1 (also named human SCM-1 alpha and ATAC) and its mouse homologue belong to the C or gamma subfamily of chemokines. The C chemokines lack two (the 1st and 3rd ) of the four invariant cysteine residues normally found in the CC and CXC chemokines and have an extended carboxy terminus. Human lymphotactin encodes a 114 amino acid residue precursor protein with a 21 amino acid residue predicted signal peptide. The expression of lymphotactin is abundant in some activated T cells such as activated CD8⁺ T cells and other class I MHC restricted T cells. Lptn expression is absent in CD4⁺ T cells. Human and mouse Lptn share approximately 60% amino acid sequence homology. The gene for lymphotactin has been mapped to chromosome 1 in both human and mouse. Recombinant human lymphotactin has been shown to have chemotactic activity for lymphocytes and NK cells. The orphan receptor GPR5 has been reported to be the specific receptor for Lptn.

References

Kennedy, J. et al. (1995) J. Immunol. 155:203.
Zlotnik, A. et al. (1999) Critical Reviews in Immunol. 19:1.

Long Name

Chemokine [C Motif] Ligand 1

Entrez Gene IDs

6375 (Human); 16963 (Mouse)

Alternate Names

ATAC; ATACSmall-inducible cytokine C1; C motif chemokine 1; chemokine (C motif) ligand 1; Cytokine SCM-1; LPTNSCM-1-alpha; LTNXC chemokine ligand 1; Lymphotactin; lymphotaxin; SCM-1 alpha; SCM-1; SCM1A; SCM-1a; SCYC1SCM1; single cysteine motif 1a; small inducible cytokine subfamily C, member 1 (lymphotactin); XCL1

Product Datasheets

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Related Research Areas

Citation for Human XCL1/Lymphotactin Biotinylated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

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