Human XIAP Antibody

Catalog #: MAB8221
1 Citation Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB8221
MAB8221-SP

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human XIAP Antibody Summary

Species Reactivity

Human

Specificity

Detects human XIAP in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) cIAP-1 or rh cIAP-2 is observed. Neutralizes human XIAP and human XIAP-BIR2 bioactivity. Does not neutralize XIAP-BIR3 or Livin bioactivity.

Source

Monoclonal Mouse IgG₁ Clone # 117318

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human XIAP
Met1-Ser497
Accession # P98170

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Immunohistochemistry

8-25 µg/mL

See below

Neutralization

Measured by its ability to neutralize Recombinant Human XIAP (2.75 µg/mL, Catalog # 822-XF) inhibition of Recombinant Human Caspase‑7 (0.012 µg/mL, Catalog # 823-C7) cleavage of the fluorogenic peptide substrate DEVD-AFC (50 µM). The Neutralization Dose (ND₅₀) is typically 10 µg/mL.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry

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XIAP in Human Brain. XIAP was detected in immersion fixed paraffin-embedded sections of human brain (cortex) using 25 µg/mL Mouse Anti-Human XIAP Monoclonal Antibody (Catalog # MAB8221) overnight at 4 °C. Tissue was stained with the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counter-stained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Neutralization

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Neutralization of XIAP Activity by Human XIAP Antibody. Recombinant Human Caspase-7 (0.012 µg/mL, Catalog # 823-C7) activity is measured in the presence of Recombinant Human XIAP (2.75 µg/mL, Catalog # 822-XF) that has been preincubated with increasing concentrations of Mouse Anti-Human XIAP Antibody (Catalog # MAB8221) The ND₅₀ is typically 10 µg/mL.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

6 months from date of receipt, -20 to -70 °C as supplied.
3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: XIAP

XIAP (X-chromosome linked inhibitor of apoptosis) is a member of the apoptosis (IAP) family of proteins that inhibit caspases. The BIR2 domain of XIAP inhibits caspase-3 and caspase-7. The ability of XIAP to inhibit caspases is prevented by SMAC/Diablo through binding to XIAP-BIR2 and -BIR3 domains.

Long Name

X-linked Inhibitor of Apoptosis

Entrez Gene IDs

331 (Human); 11798 (Mouse); 63879 (Rat)

Alternate Names

API3X-linked inhibitor of apoptosis protein; apoptosis inhibitor 3; baculoviral IAP repeat-containing 4; baculoviral IAP repeat-containing protein 4; BIRC4; BIRC4XLP2; E3 ubiquitin-protein ligase XIAP; EC 6.3.2.-; hIAP3; hIAP-3; hILP; IAP3; IAP-3; IAP-like protein; ILP; ILP1; Inhibitor of apoptosis protein 3; MIHA; XIAP; X-linked IAP; X-linked inhibitor of apoptosis

Product Datasheets

Product Datasheet

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Related Research Areas

Citation for Human XIAP Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1