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Human XPE/DDB2 Antibody

Catalog #: AF3297
7 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF3297
AF3297-SP
Detection of Human XPE/DDB2 by Western Blot.
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Citations (7)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human XPE/DDB2 Antibody Summary

Species Reactivity
Human
Specificity
Detects human XPE/DDB2 in Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human XPE/DDB2
Met1-Lys427
Accession # Q92466
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
0.5 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human XPE/DDB2 antibody by Western Blot. View Larger

Detection of Human XPE/DDB2 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and U2OS human osteosarcoma cell line. PVDF membrane was probed with 0.5 µg/mL of Human XPE/DDB2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3297) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for XPE/DDB2 at approximately 45 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: XPE/DDB2

Xeroderma Pigmentosum complementation group E (XPE) patients are defective in a heterodimer of damage-specific DNA-binding activity (DDB1/DDB2). DDB2 is a 48 kDa protein upregulated in cells exposed to ultraviolet light. XP patients exhibit hypersensitivity to ultraviolet light and have a heightened incidence of skin cancer.

Long Name
Xeroderma Pigmentosum E
Entrez Gene IDs
1643 (Human); 107986 (Mouse); 311186 (Rat)
Alternate Names
damage-specific DNA binding protein 2 (48kD); damage-specific DNA binding protein 2, 48kDa; Damage-specific DNA-binding protein 2; DDB p48 subunit; DDB2; DDBB; DNA damage-binding protein 2; FLJ34321; UV-damaged DNA-binding protein 2; UV-DDB 2; UV-DDB2; xeroderma pigmentosum group E protein; XP5; XPE/DDB2

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Citations for Human XPE/DDB2 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

7 Citations: Showing 1 - 7
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  1. Fluorescence-microscopy-based assay assessing regulatory mechanisms of global genome nucleotide excision repair in cultured cells
    Authors: Masayuki Kusakabe, Kaoru Sugasawa
    STAR Protocols
  2. XPC-PARP complexes engage the chromatin remodeler ALC1 to catalyze global genome DNA damage repair
    Authors: C Blessing, K Apelt, D van den He, C Gonzalez-L, MB Rother, M van der Wo, R González-P, A Yifrach, A Parnas, RG Shah, TT Kuo, DEC Boer, J Cai, A Kragten, HS Kim, OD Schärer, ACO Vertegaal, GM Shah, S Adar, H Lans, H van Attiku, AG Ladurner, MS Luijsterbu
    Nature Communications, 2022-08-13;13(1):4762.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  3. Histone deacetylation regulates nucleotide excision repair through an interaction with the XPC protein
    Authors: Masayuki Kusakabe, Erina Kakumu, Fumika Kurihara, Kazuki Tsuchida, Takumi Maeda, Haruto Tada et al.
    iScience
  4. Functional impacts of the ubiquitin-proteasome system on DNA damage recognition in global genome nucleotide excision repair
    Authors: W Sakai, M Yuasa-Suna, M Kusakabe, A Kishimoto, T Matsui, Y Kaneko, JI Akagi, N Huyghe, M Ikura, T Ikura, F Hanaoka, M Yokoi, K Sugasawa
    Sci Rep, 2020-11-12;10(1):19704.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  5. Regulation of liver receptor homologue-1 by DDB2 E3 ligase activity is critical for hepatic glucose metabolism
    Authors: TC Lai, MC Hu
    Sci Rep, 2019-03-28;9(1):5304.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  6. Human CRL4DDB2 ubiquitin ligase preferentially regulates post-repair chromatin restoration of H3K56Ac through recruitment of histone chaperon CAF-1
    Authors: Q Zhu, S Wei, N Sharma, G Wani, J He, AA Wani
    Oncotarget, 2017-10-17;8(61):104525-104542.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  7. DDB2 increases radioresistance of NSCLC cells by enhancing DNA damage responses
    Authors: Ning Zou, Guozhen Xie, Tiantian Cui, Amit Kumar Srivastava, Meihua Qu, Linlin Yang et al.
    Tumor Biology

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