Human ZFP90 Antibody

Catalog #: AF7870 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF7870
AF7870-SP

Detection of Human ZFP90 by Western Blot.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human ZFP90 Antibody Summary

Species Reactivity

Human

Specificity

Detects human ZFP90 in direct ELISAs and Western blots. In direct ELISAs, less than 2% cross-reactivity with recombinant mouse ZFP90 is observed.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human ZFP90
Lys94-Lys207
Accession # Q8TF47

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human ZFP90 by Western Blot. Western blot shows lysates of IMR-32 human neuroblastoma cell line and Bowes human melanoma cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human ZFP90 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7870) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for ZFP90 at approximately 72 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.2 mg/mL.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ZFP90

ZFP90 (Zinc Finger Protein 90; also ZFP756 and NK10) is a nuclear 66-72 kDa member of the ZNF505 clade, ZNF91 subfamily, kruppel C2H2-type zinc finger family of proteins. It is widely expressed, and found in cell types such as hepatocytes and cardiac myocytes. Functionally, ZFP90 acts as a transcriptional repressor by interacting with KAP-1/TRIM28/TIF1 beta. This interaction influences histone modifications and heterochromatin protein 1 deposition on DNA. Conversely, it is also known to bind to NRSF, thereby promoting NRSF sensitive gene expression. Human ZFP90 is 636 amino acids (aa) in length. It contains one KRAB domain (aa 14-85) followed by thirteen C2H2-type Zn-finger domains (aa 211-631). Over aa 94-207, human and mouse ZFP90 share 54% aa sequence identity.

Long Name

Zinc Finger Protein 90

Entrez Gene IDs

146198 (Human); 22751 (Mouse); 498945 (Rat)

Alternate Names

FoxP3-interacting Protein; KIAA1954; NK10; ZFP90; zfp-90; zinc finger protein 476; zinc finger protein 756; zinc finger protein 90 homolog (mouse); zinc finger protein 90 homolog; ZNF756