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Janelia Fluor® 549, Haloalkane

Catalog #: 8806 Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
8806/10U
Janelia Fluor® 549, Haloalkane | CAS No. 1811539-33-9 | Janelia Fluor Dyes
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Description: Cell-permeable fluorogenic fluorescent dye with a chloroalkane handle. Application: live-cell imaging as a self-labeling tag substrate

Chemical Name: 2-(3-(Azetidin-1-ium-1-ylidene)-6-(azetidin-1-yl)-3H-xanthen-9-yl)-4-((2-(2-((6-chlorohexyl)oxy)ethoxy)ethyl)carbamoyl)benzoate

Purity: ≥90%

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Biological Activity

Key Information: Janelia Fluor® 549, Haloalkane is a yellow cell-permeable fluorogenic fluorescent dye with a chloroalkane handle.

Application: Live-cell imaging as a self-labeling tag substrate. Suitable for confocal microscopy, light sheet microscropy, super resolution microscopy (SRM) techniques including dSTORM (in both live and fixed cells). Stable to fixation. Cell permeable.

Properties and Photophysical Data: Janelia Fluor® 549, Haloalkane shows a high degree of fluorogenicity. Excitation and emission maxima (λ) are 549 nm and 571 nm, respectively; quantum yield = 0.88.

Janelia Fluor is a registered trademark of Howard Hughes Medical Institute.

Optical Data for Janelia Fluor® 549, Haloalkane

Emission Color Yellow
Brightness
λabs 549 nm
λem 571 nm
Quantum Yield (φ) 0.88
Closest Laser line 532 nm
Cell Permeable Yes
Application Confocal Microscopy, Super Resolution Microscopy (SRM)

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Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

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Technical Data

M.Wt:
660.21
Formula:
C37H42ClN3O6
Solubility:
Soluble to 10 mM in DMSO
Purity:
≥90%
Storage:
Store at -20°C
CAS No:
1811539-33-9

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.

Additional Information

Licensing Caveats:
Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus

Background References

  1. A general method to fine-tune fluorophores for live-cell and in vivo imaging.
    Grimm et al.
    Nat.Methods, 2017;14:987

Product Datasheets

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