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Janelia Fluor® 585, Haloalkane

Catalog #: 8807 Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
8807/10U
Janelia Fluor® 585, Haloalkane | CAS No. 1811539-90-8 | Janelia Fluor Dyes
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Description: Cell-permeable fluorogenic fluorescent dye with a chloroalkane handle. Application: live-cell imaging as a self-labeling tag substrate

Chemical Name: 4-((2-(2-((6-Chlorohexyl)oxy)ethoxy)ethyl)carbamoyl)-2-(3-(3,3-difluoroazetidin-1-ium-1-ylidene)-6-(3,3-difluoroazetidin-1-yl)-10,10-dimethyl-3,10-dihydroanthracen-9-yl)benzoate

Purity: ≥95%

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Biological Activity

Key Information: Janelia Fluor® 585, Haloalkane is an orange cell-permeable fluorogenic fluorescent dye with a chloroalkane handle.

Application: Live-cell imaging as a self-labeling tag substrate. Suitable for confocal microscopy, light sheet microscropy, super resolution microscopy (SRM) techniques including dSTORM (in both live and fixed cells). Stable to fixation. Cell permeable.

Properties and Photophysical Data: Janelia Fluor® 585, Haloalkane shows a high degree of fluorogenicity. Excitation and emission maxima (λ) are 585 nm and 609 nm, respectively; quantum yield = 0.78; extinction coefficient = 156,000 M-1cm-1 (measured in ethanol or TFE plus 0.1% TFA); lactone-zwitterion equilibrium constant (KL-Z) <0.0001. Turn-on ratio in vitro A/A0 = 79, F/F0 = 99. Exhibits low non-specific background staining.

Janelia Fluor is a registered trademark of Howard Hughes Medical Institute.

Optical Data for Janelia Fluor® 585, Haloalkane

Janelia Fluor<sup>®</sup> 585, Haloalkane Dye Spectra
Emission Color Orange
Brightness
λabs 597 nm
λem 617 nm
Quantum Yield (φ) 0.78
Closest Laser line 561 nm
Cell Permeable Yes
Application Confocal Microscopy, Super Resolution Microscopy (SRM)

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Technical Data

M.Wt:
758.25
Formula:
C40H44ClF4N3O5
Solubility:
Soluble to 10 mM in DMSO
Purity:
≥95%
Storage:
Store at -20°C
CAS No:
1811539-90-8

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.

Additional Information

Licensing Caveats:
Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus

Background References

  1. A general method to fine-tune fluorophores for live-cell and in vivo imaging.
    Grimm et al.
    Nat.Methods, 2017;14:987

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