Jess Simple Western System
Jess Automated Western Blot System
Jess Simple Western System Summary
Meet Jess, your protein analysis problem solver. Jess automates the protein separation and immunodetection of traditional Western blotting, eliminating many of the tedious, error-prone steps. Just load your samples and reagents into the microplate, and Jess does the rest. She separates your protein by size and precisely manages antibody additions, incubations, washes and even the detection steps. Come back to fully analyzed and quantitated results in just 3 hours. With Jess’s chemiluminescent detection you’ll get picogram-level sensitivity, letting you maximize the data you get from your sample. Go further with multiplexing—her fluorescent detection gets all the information you need in one shot. Best of all, you get reliable and reproducible results with target normalization to the amount of protein loaded.
Meet Jess 
Features
Fluorescence Detection
Why bother stripping and reprobing? Maximize your time and sample, and get the information you need in one shot, by multiplexing.
Jess’s fluorescent detection capabilities enable two-color protein detection for multiplexing. Detection of Stat3 (green) and phospho-Stat3 (red) in K562 cell lysate. Lane 1: ladder; Lane 2: phospho-Stat3; Lane 3: Stat 3; Lane 4: phospho-Stat3/Stat3.
Chemiluminescence Detection
Working with low abundance targets or precious samples? Chemiluminescent detection gives you picogram-level sensitivity, letting you maximize the data you get from your sample.
Explore chemiluminescent Westerns with lane view, or dive deeper with quantitative electropherograms.
Protein Normalization
Jess gives you an easy way to see if your samples contain a consistent protein load—just load her in-capillary protein normalization reagent into her assay plate and she’ll take care of the rest. Her proprietary fluorescent protein normalization reagent measures proteins immobilized in the same capillary as your immunoassay. Best of all, Jess’s multiple detection capabilities enable two-color protein detection for multiplexing and chemiluminescent detection, on top of protein normalization.
With protein normalization, you can take your protein load comparison and transform your data to effectively normalize your samples, increasing your confidence in your data interpretation.
Blot Imaging
Still doing traditional Westerns? Snap! Get the picture with Jess’s blot imaging system.
1. Image traditional chemiluminescent blot membranes 2. Chemiluminescent Western blots imaged on Jess
Learn more at Bio-Techne.com
Resources at Bio-Techne.com
Specifications
Product Datasheets
Scientific Data
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Multiplexed analysis of total AKT and pAKT with total protein normalization using Jess. (A) Lysates from Jurkat cells either untreated (-) or treated (+) with calyculin A were analyzed at 0.25 mg/mL and 0.15 mg/mL final concentrations, respectively. Each lysate was probed for total AKT with Stellar IR (green bands) and pAKT with Stellar NIR (red bands). (B) The Stellar Total Protein Assay was simultaneously performed in the same lanes shown in panel A.
Background: Jess Simple Western System
Members of the ADAMTS family contain pro, catalytic, disintegrin-like, cysteine-rich, spacer and a variable number of TS type 1 motifs. In contrast to membrane-anchored ADAMs, ADAMTSs are secreted and are all active proteases. They are essential for aggrecan turnover and procollagen processing, regulation of which is important in many physiological and pathological processes such as angiogenesis and arthritis.
FAQs
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Easy to use, very short learning curve. Can achieve data quickly with little hands-on time. Software has many useful features for data analysis. Best part is the low sample volume that is required.
