Home / Compounds for ESC/iPSC Proliferation and Renewal / Kyoto Probe-1

Kyoto Probe-1

Catalog #: 7419 Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
7419/100U
Kyoto Probe-1 | CAS No. 2088021-81-0 | Compounds for ESC/iPSC Proliferation and Renewal
1 Image
Description: Fluorescent probe that selectively identifies undifferentiated iPS/ES cells
Alternative Names: KP1

Chemical Name: 9-(4-Fluorophenyl)-3-imino-3H-xanthen-6-amine trifluoroacetate

Purity: ≥95%

Product Details
Reviews
Biological Activity Technical Data Additional Information Product Datasheets Calculators

Biological Activity

Kyoto Probe-1 distinguishes between undifferentiated iPS/ES cells and differentiated cells, so may be used for monitoring pluripotency during hESC or iPSC maintenance. Selectivity of labeling is due to ATP-binding cassette transporters ABCB1 and ABCG2 - these mediate efflux of Kyoto Probe-1 and are expressed in differentiated cells, but are repressed in hiPSC and hESC. Kyoto Probe-1 distinguishes between SSEA-4-positive hESCs and human early hematopoietic cells expressing CD45, CD235, CD41a, or CD43 in flow cytometry experiments, making it a valuable tool for studying early hematopoiesis. The product also distinguishes between hiPSCs and hiPSC-derived cardiomyocytes. Note that Kyoto Probe-1 does not differentiate between hiPSCs and differentiated human neuronal stem cells.

Kyoto Probe-1 localizes to the mitochondria in undifferentiated iPS/ES cells only. Suitable in flow cytometry and live cell imaging.

Excitation maximum = 515 nm; emission maximum = 529 nm; Quantum yield = 0.45

Optical Data for Kyoto Probe-1

Kyoto Probe-1 Dye Spectra
Emission Color Green
λabs 515 nm
λem 529 nm
Quantum Yield (φ) 0.45
Cell Permeable Yes
Application Flow Cytometry, Live-cell Imaging

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

Spectral Viewer

Spectral Viewer

Technical Data

M.Wt:
418.35
Formula:
C19H13FN2O.CF3CO2H
Solubility:
Soluble to 100 mM in DMSO
Purity:
≥95%
Storage:
Store at -20°C
CAS No:
2088021-81-0

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.

Additional Information

Licensing Caveats:
Sold under license from New York University

Product Datasheets

Reconstitution Calculator
Molarity Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Molarity Calculator

=
x
x
g/mol

*When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and CoA (available online).

FAQs

No product specific FAQs exist for this product, however you may

View all Small Molecule FAQs

Reviews for Kyoto Probe-1

There are currently no reviews for this product. Be the first to review Kyoto Probe-1 and earn rewards!

Have you used Kyoto Probe-1?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review
Tocris Bioscience is the leading supplier of novel and exclusive tools for life science research with over 30 years' experience in the industry. Tocris is a Bio-Techne brand.