Llama IgG APC-conjugated Antibody

Catalog #: F011126 Datasheet / COA / SDS

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F011126

Detection of Siglec-3/CD33 in HEK293 Human Cell Line Transfected with Human Siglec-3/CD33 and eGFP by Flow Cytometry.

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All IgG Antibodies

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Llama IgG APC-conjugated Antibody Summary

Species Reactivity

Llama

Specificity

Detects Llama IgG in direct ELISAs.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

Allophycocyanin (Excitation= 620-650 nm, Emission= 660-670 nm)

Applications

Recommended Concentration

Sample

Flow Cytometry

10 µL/10⁶ cells

Designed for use as a secondary developing reagent in immunofluorescent assays, such as flow cytometry, where the primary antibody does not have a fluorescent reporter, is of llama origin, and is of IgG class. The recommended concentration is 10 μL/106 cells.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of Siglec-3/CD33 in HEK293 Human Cell Line Transfected with Human Siglec-3/CD33 and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with human Siglec-3/CD33 and eGFP was stained with (A) Llama Anti-Human Siglec-3/CD33 Llamabody VHH Monoclonal Antibody (LMAB10733) or (B) normal llama IgG (AF011) followed by APC-conjugated Goat-anti-llama IgG Secondary Antibody (F011126). Staining was performed using our Staining Membrane-associated Proteins protocol.

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Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: IgG

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.

Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

Long Name

Immunoglobulin G

Alternate Names

IgG; Immunoglobulin G; ImmunoglobulinG