Key Product Details

Validated by

Knockout/Knockdown, Biological Validation

Species Reactivity

Validated:

Human, Mouse, Rat, Porcine

Cited:

Human, Mouse, Rat, Porcine, Feline

Predicted:

Bovine (92%), Chicken (92%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Simple Western, Knockdown Validated

Cited:

Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Lysyl Oxidase/LOX Primary Antibodies »

Product Specifications

Immunogen

A synthetic peptide made to an internal region of the human LOX protein (within residues 200-300). [Swiss-Prot P28300].

Reactivity Notes

Rat reactivity reported in scientific literature (PMID: 27169768). Immunogen displays the following percentage of sequence identity for non-tested species: bovine (92%), chicken (92%), xenopus (85%). Porcine reactivity reported in scientific literature (PMID: 30036726).

Localization

Secreted, extracellular space.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for LOX Antibody - BSA Free

Western Blot: LOX Antibody [NB100-2527]

Western Blot: LOX Antibody [NB100-2527]

LOX-Antibody-Western-Blot-NB100-2527-img0015.jpg
Immunohistochemistry-Paraffin: LOX Antibody [NB100-2527]

Immunohistochemistry-Paraffin: LOX Antibody [NB100-2527]

LOX-Antibody-Immunohistochemistry-Paraffin-NB100-2527-img0017.jpg
Simple Western: LOX Antibody [NB100-2527]

Simple Western: LOX Antibody [NB100-2527]

Simple Western: LOX Antibody [NB100-2527] - Simple Western shows a specific band for LOX in 1.0 mg/ml of HeLa cell lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.
Immunohistochemistry-Paraffin: LOX Antibody [NB100-2527]

Immunohistochemistry-Paraffin: LOX Antibody [NB100-2527]

Immunohistochemistry-Paraffin: LOX Antibody [NB100-2527] - LOX in fibrotic mice lung tissue. Primary antibody at 1:100, incubated overnight at 4C. Image from verified customer review.
Knockdown Validated: LOX Antibody [NB100-2527]

Western Blot LOX Antibody [NB100-2527]

LOX-Antibody-Knockdown-Validated-NB100-2527-img0016.jpg
Immunocytochemistry/ Immunofluorescence: LOX Antibody [NB100-2527]

Immunocytochemistry/ Immunofluorescence: LOX Antibody [NB100-2527]

Immunocytochemistry/Immunofluorescence: LOX Antibody [NB100-2527] - LOX antibody was tested in HeLa cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).
Immunohistochemistry: LOX Antibody [NB100-2527]

Immunohistochemistry: LOX Antibody [NB100-2527]

LOX-Antibody-Immunohistochemistry-NB100-2527-img0014.jpg
Immunocytochemistry/ Immunofluorescence: LOX Antibody [NB100-2527]

Immunocytochemistry/ Immunofluorescence: LOX Antibody [NB100-2527]

Immunocytochemistry/Immunofluorescence: LOX Antibody [NB100-2527] - Staining in human fibroblasts. Image from verified customer review.
Western Blot: LOX Antibody [NB100-2527]

Western Blot: LOX Antibody [NB100-2527]

Western Blot: LOX Antibody [NB100-2527] - Analysis of LOX in human kidney using NB100-2527.
Immunohistochemistry: LOX Antibody [NB100-2527]

Immunohistochemistry: LOX Antibody [NB100-2527]

Immunohistochemistry: LOX Antibody [NB100-2527] - Staining of LOX in mouse stomach.
LOX Antibody

Western Blot: LOX Antibody [NB100-2527] -

Western Blot: LOX Antibody [NB100-2527] - Patupilone counteracts hypoxia- but not IR-induced LOX-secretion. (A) Western blot of basal & IR-induced LOX in conditioned media derived from patupilone-untreated or patupilone-pretreated (0.5 nM) cells & quantification of band intensities from 3 independent experiments. (B) Western blot of basal & hypoxia-induced LOX in conditioned media derived from patupilone-untreated or patupilone-pretreated (0.5 nM) cells & quantification of band intensities from 3 independent experiments. (C) LOX gene transcription 16 h after irradiation & hypoxia, determined by RT-PCR, averaged over 3 independent experiments. Image collected & cropped by CiteAb from the following publication (https://bmccancer.biomedcentral.com/articles/10.1186/1471-2407-14-532), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for LOX Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:100-1:250

Immunohistochemistry-Paraffin

1:100-1:250

Simple Western

1:25

Western Blot

0.5-4ug/ml
Application Notes

This LOX antibody is useful for Immunocytochemistry/Immunofluorescence, Immunohistochemistry-paraffin embedded sections, and Western blot. In Western blot, bands are observed ~58 kDa representing glycosylated Lox and ~32 kDa representing the mature, secreted form of Lox. In ICC/IF nuclear staining was observed in HeLa cells, which is expected for the mature form of LOX according to published literature (PMID 17287363 and 10996848).

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point.
See Simple Western Antibody Database for Simple Western validation: Tested in HeLa lysate 1.0 mg/mL, separated by Size, antibody dilution of 1:25, apparent MW was 27 kDa. Separated by Size-Wes, Sally Sue/Peggy Sue.

Reviewed Applications

Read 5 reviews rated 4 using NB100-2527 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Lysyl Oxidase/LOX

LOX (lysyl oxidase) is a copper-dependent amine oxidase enzyme responsible for post-translational oxidative deamination of peptidyl lysine residues in precursors to fibrous collagen and elastin. LOX is synthesized as an inactive 50 kD proenzyme that is N-glycosylated in ER/Golgi complex and then secreted into the extracellular environment, where it is processed by pro-collagen C-proteinases (mammalian Tolloids and BMP-1) into an active enzyme (28-32 kD) and an 18 kD propeptide (LOX-PP) which can be N-glycosylated intracellularly into a ~35 kD form. LOX activity is critical in maintaining the tensile as well as elastic features of the connective tissues of skeletal, pulmonary and cardiovascular systems. LOX deficiency leads to intra-gestational lethality in LOX-/- mice wherein the pups exhibits aortic aneurysms/tortuosity/rupture. LOX expression is highly regulated during normal development. Aberrant expression or activity has been documented in a range of diseases predominantly associated with the ECM such as Cutis laxa or OHS (occipital horn syndrome), Menkes' syndrome, myocardial ischaemia/heart failure, atherosclerosis, scleroderma, liver cirrhosis, Pseudoexfoliation syndrome and glaucoma, Alzheimer's/non-Alzheimer's dementia, Wilson's disease, primary biliary cirrhosis as well as in many type of cancers, wherein LOX implicates in promoting tumor cell invasion and metastasis.

Long Name

Lysyl Oxidase

Alternate Names

AAT10

Gene Symbol

LOX

Additional Lysyl Oxidase/LOX Products

Product Documents for LOX Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for LOX Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for LOX Antibody - BSA Free

Customer Reviews for LOX Antibody - BSA Free (5)

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Customer Images

Showing  1 - 5 of 5 reviews Showing All
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  • LOX Antibody
    Name: Wan-Ching Cheng
    Application: Immunohistochemistry-Paraffin
    Sample Tested: IHC-P Sample Tested
    Species: cat and Rat
    Verified Customer | Posted 08/03/2018
    LOX Antibody - BSA Free NB100-2527
  • LOX Antibody
    Name: Anonymous
    Application: Immunofluorescence
    Sample Tested: fibroblasts
    Species: Human
    Verified Customer | Posted 06/07/2018
    LOX Antibody - BSA Free NB100-2527
  • Name: Anonymous
    Application: Immunohistochemistry-Paraffin
    Sample Tested: mouse lung
    Species: Mouse
    Verified Customer | Posted 01/14/2015
    LOX immunoreactivity in fibrotic mice lung
    LOX Antibody - BSA Free NB100-2527
  • Name: Saray Varona
    Application: Western Blot
    Sample Tested: mouse vascular smooth muscle cell
    Species: Mouse
    Verified Customer | Posted 03/14/2014
    LOX protein levels analyzed in cell lysates and supernatants from mice vascular smooth muscle cells
    LOX Antibody - BSA Free NB100-2527
  • Name: Anonymous
    Application: Western Blot
    Sample Tested: Renal cell carcinoma cell line UMRC2, Sample Amount: 100ug
    Species: Human
    Verified Customer | Posted 06/19/2009

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Protocols

View specific protocols for LOX Antibody - BSA Free (NB100-2527):

LOX Antibody:
Antigen unmasking

1. Bring slides to a boil in 10 mM sodium citrate buffer pH 6.0 then maintain at a sub-boiling temperature for 10 minutes.
2. Cool slides on bench top for 30 minutes.

Staining

1. Wash sections in dH2O three times for 5 minutes each.
2. Wash section in wash buffer (1X PBS/0.1% Tween-20 (1X PBST)) for 5 minutes.
3. Block each section with 100-400ul blocking solution (1X PBST, 5% goat serum) for 1 hour at room temperature.
4. Remove blocking solution and add 100-400 ul primary antibody diluted in 1X PBST, 5% goat serum to each section.
5. Incubate overnight at 4C.
6. Remove antibody solution and wash sections in wash buffer three times for 5 minutes each.
7. Add 100-400 ul biotinylated secondary antibody, diluted in 1X PBST, 5% goat serum.
8. Incubate 30 minutes at room temperature.
9. Remove secondary antibody solution and wash sections three times with wash buffer for 5 minutes each.
10. Add 100-400 ul Streptavidin HRP reagent to each section and incubate for 30 minutes at room temperature.
11. Wash sections three times in wash buffer for 5 minutes each.
12. Add 100-400 ul DAB substrate to each section and monitor staining closely.
13. As soon as the sections develop, immerse slides in dH2O.
14. Counterstain sections in hematoxylin.
15. Wash sections in dH2O two times for 5 minutes each.
16. Dehydrate sections.
17. Mount coverslips.

LOX Antibody:
1. Perform SDS-PAGE (4-12%, Bis-Tris) on samples to be analyzed, loading 40 ug of total protein per lane.
2. Transfer proteins to Nitrocellulose according to the instructions provided by the manufacturer of the transfer apparatus.
3. Rinse membrane with dH2O and then stain the blot using ponceau S for 1-2 minutes to access the transfer of proteins onto the nitrocellulose membrane. Rinse the blot in water to remove excess stain and mark the lane locations and locations of molecular weight markers using a pencil.
4. Rinse the blot in TBS for approximately 5 minutes.
5. Block the membrane using 5% non-fat dry milk + 1% BSA in TBS, overnight at 4C.
6. Rinse the membrane in dH2O and then wash the membrane in wash buffer [TBS + 0.1% Tween] 3 times for 10 minutes each.
7. Dilute the rabbit anti-LOX primary antibody (NB 100-2527) in blocking buffer and incubate 1 hour at room temperature.
8. Rinse the membrane in dH2O and then wash the membrane in wash buffer [TBS + 0.1% Tween] 3 times for 10 minutes each.
9. Apply the diluted rabbit-IgG HRP-conjugated secondary antibody in blocking buffer (as per manufacturer's instructions) and incubate 1 hour at room temperature.
10. Wash the blot in wash buffer [TBS + 0.1% Tween] 3 times for 10 minutes each (this step can be repeated as required to reduce background).
11. Apply the detection reagent of choice in accordance with the manufacturer's instructions (Pierce's ECL).

**Note: Tween-20 can be added to the blocking or antibody dilution buffer at a final concentration of 0.05-0.2%, provided it does not interfere with antibody-antigen binding.

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for LOX Antibody - BSA Free

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  • Q: Hi, I was wondering if you have any blocking peptides for NB100-59729, NB100-2527 and NB100-2530?

    A: Catalog # NB100-2527PEP is the blocking peptide for catalog # NB100-2527.

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