Lysis Buffer 6 (1 x 21 mL) Summary
- Store unopened vial at 2-8 °C. Stable for up to 30 days after opening when stored at 2-8 °C.
- For use with recommended Proteome Profiler™ Array kits. Please refer to the product-specific package insert for more information.
Kit Contents
1 vial (21 mL) of a denaturing buffered solution.
Specifications
Source
N/A
Shipping Conditions
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Applications
ELISA
Product Datasheets
Citations for Lysis Buffer 6 (1 x 21 mL)
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
15
Citations: Showing 1 - 10
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A small-molecule PI3K? activator for cardioprotection and neuroregeneration
Authors: Gong, GQ;Bilanges, B;Allsop, B;Masson, GR;Roberton, V;Askwith, T;Oxenford, S;Madsen, RR;Conduit, SE;Bellini, D;Fitzek, M;Collier, M;Najam, O;He, Z;Wahab, B;McLaughlin, SH;Chan, AWE;Feierberg, I;Madin, A;Morelli, D;Bhamra, A;Vinciauskaite, V;Anderson, KE;Surinova, S;Pinotsis, N;Lopez-Guadamillas, E;Wilcox, M;Hooper, A;Patel, C;Whitehead, MA;Bunney, TD;Stephens, LR;Hawkins, PT;Katan, M;Yellon, DM;Davidson, SM;Smith, DM;Phillips, JB;Angell, R;Williams, RL;Vanhaesebroeck, B;
Nature 2023-06-01
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Resistance to tyrosine kinase inhibitors promotes renal cancer progression through MCPIP1 tumor-suppressor downregulation and c-Met activation
Authors: P Marona, J Górka, O Kwapisz, J Jura, J Rys, RM Hoffman, K Miekus
Cell Death & Disease, 2022-09-22;13(9):814. 2022-09-22
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PI3K/Akt pathway mediates the positive inotropic effects of insulin in Langendorff-perfused rat hearts
Authors: Y Nakadate, A Kawakami, H Sato, T Sato, T Oguchi, K Omiya, T Matsuoka, T Schricker, T Matsukawa
Oncogene, 2022-06-13;12(1):9793. 2022-06-13
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Degradation and Inhibition of Epigenetic Regulatory Protein BRD4 Exacerbate Alzheimer's Disease-Related Neuropathology in Cell Models
Authors: S Zhang, P Bai, D Lei, Y Liang, S Zhen, G Bakiasi, H Pang, SH Choi, C Wang, RE Tanzi, C Zhang
The Journal of Biological Chemistry, 2022-03-03;298(4):101794. 2022-03-03
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Genome wide CRISPR/Cas9 screen identifies the coagulation factor IX (F9) as a regulator of senescence
Authors: P Carpintero, M Borghesan, O Eleftheria, B Pan-Castil, JA Fafián-Lab, TP Mitchell, A Yuste, M Ogrunc, TD Nightingal, M Mayan, A O'Loghlen
Cell Death & Disease, 2022-02-19;13(2):163. 2022-02-19
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PARP1 Inhibitor and Trabectedin Combination Does Not Increase Tumor Mutational Burden in Advanced Sarcomas-A Preclinical and Translational Study
Authors: Y Pignochino, G Crisafulli, G Giordano, A Merlini, E Berrino, ML Centomo, G Chiabotto, S Brusco, M Basiricò, E Maldi, A Pisacane, V Leuci, D Sangiolo, L D'Ambrosio, M Aglietta, B Kasper, A Bardelli, G Grignani
Cancers, 2021-12-15;13(24):. 2021-12-15
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EphA2 Expression in Bone Sarcomas: Bioinformatic Analyses and Preclinical Characterization in Patient-Derived Models of Osteosarcoma, Ewing's Sarcoma and Chondrosarcoma
Authors: G Giordano, A Merlini, G Ferrero, G Mesiano, E Fiorino, S Brusco, ML Centomo, V Leuci, L D'Ambrosio, M Aglietta, D Sangiolo, G Grignani, Y Pignochino
Cells, 2021-10-26;10(11):. 2021-10-26
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Androgen Receptor Activation in Glioblastoma Can Be Achieved by Ligand-Independent Signaling through EGFR-A Potential Therapeutic Target
Authors: N Zalcman, M Gutreiman, T Shahar, M Weller, I Lavon
International Journal of Molecular Sciences, 2021-10-11;22(20):. 2021-10-11
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Epithelial-mesenchymal transition induced by SARS-CoV-2 required transcriptional upregulation of Snail
Authors: YJ Lai, CH Chao, CC Liao, TA Lee, JM Hsu, WC Chou, J Wang, HC Huang, SJ Chang, YL Lin, CW Li
American journal of cancer research, 2021-05-15;11(5):2278-2290. 2021-05-15
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Priming with HDAC Inhibitors Sensitizes Ovarian Cancer Cells to Treatment with Cisplatin and HSP90 Inhibitors
Authors: AJ Rodrigues, JJ Bandolik, FK Hansen, T Kurz, A Hamacher, MU Kassack
Int J Mol Sci, 2020-11-05;21(21):. 2020-11-05
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Small Extracellular Vesicles Are Key Regulators of Non-cell Autonomous Intercellular Communication in Senescence via the Interferon Protein IFITM3
Authors: M Borghesan, J Fafián-Lab, O Eleftheria, P Carpintero, M Paez-Ribes, G Vizcay-Bar, A Swisa, D Kolodkin-G, P Ximénez-Em, R Lowe, B Martín-Mar, H Peinado, J Muñoz, RA Fleck, Y Dor, I Ben-Porath, A Vossenkamp, D Muñoz-Espi, A O'Loghlen
Cell Rep, 2019-06-25;27(13):3956-3971.e6. 2019-06-25
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Targeting CREB-binding protein overrides LPS induced radioresistance in non-small cell lung cancer cell lines
Authors: MY Gökyildiri, U Grandel, K Hattar, G Dahlem, E Schuetz, FH Leinberger, F Eberle, U Sibelius, F Grimminger, W Seeger, R Engenhart-, E Dikomey, FSB Subtil
Oncotarget, 2018-06-22;9(48):28976-28988. 2018-06-22
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miR-30e* is overexpressed in prostate cancer and promotes NF-?B-mediated proliferation and tumor growth
Authors: SM Egan, E Karasik, L Ellis, SO Gollnick
Oncotarget, 2017-06-28;8(40):67626-67638. 2017-06-28
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L1 Cell Adhesion Molecule Promotes Migration and Invasion via JNK Activation in Extrahepatic Cholangiocarcinoma Cells with Activating KRAS Mutation
Authors: H Kim, H Hwang, H Lee, HJ Hong
Mol. Cells, 2017-05-24;40(5):363-370. 2017-05-24
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Glycemia and the cardioprotective effects of insulin pre-conditioning in the isolated rat heart
Authors: Y Nakadate, H Sato, T Oguchi, T Sato, A Kawakami, T Ishiyama, T Matsukawa, T Schricker
Cardiovasc Diabetol, 2017-04-04;16(1):43. 2017-04-04
FAQs
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Does Lysis Buffer 6 in the Proteome Profiler™ Array contain phosphatase and protease inhibitors?
Lysis Buffer 6 contains phosphatase inhibitors but not protease inhibitors. If desired, protease inhibitors can be added to the lysis buffer immediately prior to use. We recommend 10 μg/ml Aprotinin (Tocris; Catalog # 4139), 10 μg/ml Leupeptin (Tocris; Catalog # 1167), and 10 μg/ml Peptstatin (Tocris; Catalog # 1190).
Reviews for Lysis Buffer 6 (1 x 21 mL)
Average Rating: 5 (Based on 3 Reviews)
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Lysis Buffer 6 (1 x 21 mL)
By Anonymous
on 04/16/2021
Great for downstream analysis of phospho-proteins. Higher protein yield than RIPA with small amounts of cells.
Lysis Buffer 6 (1 x 21 mL)
By Danlee Enzler
on 04/18/2018
Lysis Buffer 6 (1 x 21 mL)
By Leslie Priddy
on 04/01/2018
