MitoBrilliant™ 646
Purity: ≥95%
Biological Activity
MitoBrilliant probes have been developed to overcome some common limitations encountered with standard mitochondrial trackers, offering clearer answers to scientific questions. They are next-generation fluorescent stains for the localization and tracking of mitochondria in both live and fixed cells. The range harnesses Janelia Fluor® dye technology, conferring some of the properties that make these widely used dyes, into mitochondrial stains.Key information: MitoBrilliant™ 646 is a red-fluorescent mitochondrial stain for both live and fixed cells.
Used for: mitochondrial staining for both live and pre-fixed cells/tissue.
Application: flow cytometry, super-resolution microscopy-STED, high content screening, IHC/ICC.
Properties and Photophysical Data: MitoBrilliant™ 646 is retained in mitochondria following fixation with exceptionally clear staining and is retained in the mitochondria of live cells following loss of the mitochondrial membrane potential. Excitation and emission maxima (λ) are 655 nm and 668 nm, respectively; extinction coefficient = 125,000 M-1cm-1.
Two dyes that accumulate in the mitochondria of live cells in a mitochondrial membrane potential (Δψm) dependent manner are also available: MitoBrilliant™ Live 646 (red emission) and MitoBrilliant™ Live 549 (yellow/orange emission).
Please refer to the protocol for guidelines on product use, and download the MitoBrilliant Product Guide to view data for each of the dyes in different applications.
MitoBrilliant is a trademark of Bio-Techne Corp.
Scientific Data
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Live-cell image of mitochondria stained with MitoBrilliant™ 646 HeLa cells were incubated with MitoBrilliant™ 646 (100 nM) for 40 minutes and counterstained with DAPI (Catalog # 5748). Image was taken using an LSM880 Confocal using a 63x objective. Scale bar = 10 µm.
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Fixed-cell image of mitochondria stained with MitoBrilliant™ 646. Dermal fibroblast cells were incubated with MitoBrilliant™ 646 (100 nM) for 1 hour at 37°C, fixed with ice-cold acetone-methanol (1:1) and counterstained with Hoechst 33342 (Catalog # 5117). Image was taken using an Echo Revolve microscope using a 20x objective. Scale bar = 20 µm.
| Product Name | Core dye structure | Abs/Em (nm) | Δψm dependent | Live/Fixed cell use | Stain pre-fixed cells? | Image without wash step | Demonstrated Applications | MitoBrilliant™ 646 Cat. No. 7700 | Janelia Fluor® technology | 655/668 | No* | Suitable for both live and fixed cell work | Yes | Yes, but replacing media recommended | Fixed-cell imaging, Live-cell imaging, Flow Cytometry, IHC/ICC, Super-resolution microscopy – STED, High-content screening |
|---|---|---|---|---|---|---|---|
| MitoBrilliant™ Live 646 Cat. No. 7417 | Janelia Fluor® technology | 648/662 | Yes | Live-cell work only | No | Yes, but replacing media recommended | Live-cell imaging, Flow Cytometry, High-content screening |
| MitoBrilliant™ Live 549 Cat. No. 7693 | Janelia Fluor® technology | 550/568 | Yes | Live-cell work only | No | Yes, but replacing media recommended | Live-cell imaging, Flow Cytometry, High-content screening |
Optical Data for MitoBrilliant™ 646
| Emission Color | Red |
|---|---|
| λabs | 655 nm |
| λem | 668 nm |
| Extinction Coefficient (ε) | 125000 M-1cm-1 |
| Closest Laser line | 640 nm |
| Application | Fixed-cell imaging, Live-cell imaging, Flow Cytometry, IHC/ICC, Super-resolution microscopy – STED, |
Plan Your Experiments
Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.
Spectral Viewer
Technical Data
The technical data provided above is for guidance only.
For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Product Datasheets
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FAQs
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