Mouse 5'-Nucleotidase/CD73 Antibody

Catalog #: MAB4488 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB4488
MAB4488-SP

Detection of 5'‑Nucleotidase/CD73 in Mouse Splenocytes by Flow Cytometry.

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Mouse 5'-Nucleotidase/CD73 Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse 5'‑Nucleotidase/CD73 in direct ELISAs.

Source

Monoclonal Rat IgG_2A Clone # 496406

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant mouse 5'‑Nucleotidase/CD73
Trp29-Lys549
Accession # Q61503

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Flow Cytometry

0.25 µg/10⁶ cells

See below

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of 5'‑Nucleotidase/CD73 in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with Rat Anti-Mouse CD3 APC-conjugated Monoclonal Antibody (Catalog # FAB4841A) and either (A) Rat Anti-Mouse 5'-Nucleotidase/CD73 Monoclonal Antibody (Catalog # MAB4488) or (B) Rat IgG_2AIsotype Control (Catalog # MAB006) followed by Phycoerythrin-conjugated Anti-Rat IgG F(ab')₂Secondary Antibody (Catalog # F0105B). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: 5'-Nucleotidase/CD73

CD73, an ecto-5’-Nucleotidase, is an ectoenzyme that is attached to the cell membrane by a glycosyl phophatidylinositol anchor (1, 2). The enzyme is expressed by most cell types. The 5’-Nucleotidase activity of CD73 converts extracellular nucleoside-5’-monophosphates to nucleosides. CD73 is one of several enzymes responsible for the production of extracellular adenosine, a signaling molecule that is involved in responses to inflammation and tissue injury (3).

References

Resta, R. et al. (1993) Gene 133:171.
Resta, R. et al. (1998) Immunol. Rev. 161:95.
Pilcher, M. et al. (2003) J. Biol. Chem. 278:13468.

Entrez Gene IDs

4907 (Human); 23959 (Mouse); 58813 (Rat); 102114976 (Cynomolgus Monkey)

Alternate Names

5' nucleotidase (CD73); 5'-NT; 5-NT; 5'-Nucleotidase; 5'-nucleotidase, ecto (CD73); CD73 antigen; CD73; E5NT; EC 3.1.3.5; ecto-5'-nucleotidase; eN; eNT; NT; NT55'-nucleotidase; NT5E; NTE; Purine 5-Prime-Nucleotidase