Human/Mouse/Rat/Hamster ACE-2 Antibody

Catalog #: MAB3437
7 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB3437
MAB3437-SP

Detection of Human ACE‑2 by Western Blot.

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All ACE-2 Antibodies

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Citations (7)

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Human/Mouse/Rat/Hamster ACE-2 Antibody Summary

Species Reactivity

Human, Mouse, Rat, Hamster

Specificity

Detects mouse ACE-2 in direct ELISAs and human, mouse, rat and hamster ACE-2 in Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant mouse ACE-1 is observed.

Source

Monoclonal Rat IgG_2B Clone # 460502

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant mouse ACE-2
Gln18-Thr740 (predicted)
Accession # Q8R0I0

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

0.5 µg/mL

Human kidney and recombinant human ACE-2 protein, recombinant mouse ACE-2 protein, recombinant rat ACE-2 protein, and recombinant hamster ACE-2 protein

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human ACE‑2 by Western Blot. Western blot shows lysates of human kidney. PVDF membrane was probed with 0.5 µg/mL of Rat Anti-Human/Mouse/Rat/Hamster ACE‑2 Monoclonal Antibody (Catalog # MAB3437) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (HAF005). A specific band was detected for ACE‑2 at approximately 120 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

Western Blot

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Detection of Human, Mouse, Rat, and Hamster ACE‑2 by Western Blot. Western blot shows recombinant human ACE-2 protein, recombinant mouse ACE-2 protein, recombinant rat ACE-2 protein, and recombinant hamster ACE-2 protein. PVDF membrane was probed with 0.5 µg/mL of Rat Anti-Human/Mouse/Rat/Hamster ACE‑2 Monoclonal Antibody (Catalog # MAB3437) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (HAF005). A specific band was detected for ACE‑2 at approximately 120 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ACE-2

ACE-2, also called ACEH (ACE homologue), is an integral membrane protein and a zinc metalloprotease of the ACE family that also includes somatic and germinal ACE (1). Mouse ACE-2 has about 40% amino acid identity to the N- and C-terminal domains of mouse somatic ACE. The predicted mouse ACE-2 protein sequence consists of 798 amino acids, including a N-terminal signal peptide, a single catalytic domain, a C-terminal membrane anchor, and a short cytoplasmic tail. ACE-2 cleaves angiotensins I and II as a carboxypeptidase. ACE-2 mRNA is found at high levels in testis, kidney and heart and at moderate levels in colon, small intestine and ovary. Classical ACE inhibitors such as captopril and lisinopril do not inhibit ACE-2 activity. Novel peptide inhibitors of ACE-2 do not inhibit ACE activity (2). Genetic data from Drosophila, mice and rats show that ACE-2 is an essential regulator of heart function in vivo (3). In addition, ACE-2 is a key SARS-CoV Spike protein receptor in vivo and has a critical function in acute lung injury (4, 5).

References

Tipnis, S.R. et al. (2000) J. Biol. Chem. 275:33238.
Crackower, M.A. et al. (2002) Nature 417:822.
Huang, L. et al. (2003) J. Biol. Chem. 278:15532.
Kuba, K. et al. (2005) Nature Med. 11:875.
Ima, Y. et al. (2005) Nature 436:112.

Long Name

Angiotensin I Converting Enzyme 2

Entrez Gene IDs

59272 (Human); 70008 (Mouse); 302668 (Rat); 100144303 (Porcine); 480847 (Canine); 418623 (Chicken); 102130864 (Cynomolgus Monkey); 554349 (Feline); 101673097 (Ferret); 101823817 (Hamster); 108390919 (Malayan Pangolin)

Alternate Names

ACE2; ACE-2; ACEH; ACEHangiotensin I converting enzyme 2; ACE-related carboxypeptidase; angiotensin I converting enzyme (peptidyl-dipeptidase A) 2; angiotensin-converting enzyme 2; Angiotensin-converting enzyme homolog; DKFZp434A014; EC 3.4.17; EC 3.4.17.23; Metalloprotease MPROT15

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Citations for Human/Mouse/Rat/Hamster ACE-2 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

7 Citations: Showing 1 - 7
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Age-dependent regulation of SARS-CoV-2 cell entry genes and cell death programs correlates with COVID-19 disease severity
Authors: Zintis Inde, Clarence Yapp, Gaurav N. Joshi, Johan Spetz, Cameron Fraser, Brian Deskin et al.
bioRxiv
Gut microbiota-derived metabolites confer protection against SARS-CoV-2 infection
Authors: Julia A. Brown, Katherine Z. Sanidad, Serena Lucotti, Carolin M. Lieber, Robert M. Cox, Aparna Ananthanarayanan et al.
Gut Microbes
Characterization of SARS-CoV-2 Spike mutations important for infection of mice and escape from human immune sera
Authors: R Rathnasing, S Jangra, C Ye, A Cupic, G Singh, C Martínez-R, LCF Mulder, T Kehrer, S Yildiz, A Choi, ST Yeung, I Mena, V Gillespie, J De Vrieze, S Aslam, D Stadlbauer, DA Meekins, CD McDowell, V Balaraman, MJ Corley, JA Richt, BG De Geest, L Miorin, PVI study, F Krammer, L Martinez-S, V Simon, A García-Sas, M Schotsaert
Nature Communications, 2022-07-07;13(1):3921.
Species: African Green Monkey
Sample Types: Cell Lysates
Applications: Western Blot
Interferon-alpha or -beta facilitates SARS-CoV-2 pulmonary vascular infection by inducing ACE2
Authors: Timothy Klouda, Yuan Hao, Hyunbum Kim, Jiwon Kim, Judith Olejnik, Adam J. Hume et al.
Angiogenesis
SARS-CoV-2 Causes Lung Infection without Severe Disease in Human ACE2 Knock-In Mice
Authors: Emma S. Winkler, Rita E. Chen, Fahmida Alam, Soner Yildiz, James Brett Case, Melissa B. Uccellini et al.
Journal of Virology
The N501Y mutation in SARS-CoV-2 spike leads to morbidity in obese and aged mice and is neutralized by convalescent and post-vaccination human sera
Authors: R Rathnasing, S Jangra, A Cupic, C Martínez-R, LCF Mulder, T Kehrer, S Yildiz, A Choi, I Mena, J De Vrieze, S Aslam, D Stadlbauer, DA Meekins, CD McDowell, V Balaraman, JA Richt, BG De Geest, L Miorin, F Krammer, V Simon, A García-Sas, M Schotsaert
medRxiv : the preprint server for health sciences, 2021-01-20;0(0):.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot
Angiotensin-converting enzyme 2 protects from lethal avian influenza A H5N1 infections.
Authors: Zou, Zhen, Yan, Yiwu, Shu, Yuelong, Gao, Rongbao, Sun, Yang, Li, Xiao, Ju, Xiangwu, Liang, Zhu, Liu, Qiang, Zhao, Yan, Guo, Feng, Bai, Tian, Han, Zongshen, Zhu, Jindong, Zhou, Huandi, Huang, Fengming, Li, Chang, Lu, Huijun, Li, Ning, Li, Dangshen, Jin, Ningyi, Penninger, Josef M, Jiang, Chengyu
Nat Commun, 2014-05-06;5(0):3594.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot