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Mouse ADAM10 Ectodomain PE-conjugated Antibody

Catalog #: FAB946P
5 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
FAB946P-100UG
Detection of ADAM10 in Mouse Splenocytes by Flow Cytometry.
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Citations (5)
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Mouse ADAM10 Ectodomain PE-conjugated Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse ADAM10 in direct ELISAs and Western blots. In direct ELISAs, approximately 25% cross-reactivity with recombinant human (rh) rhADAM10 is observed and no cross-reactivity with rhADAM8, recombinant mouse (rm) ADAM9, rmADAM15, rhBACE, or rhTACE is observed.
Source
Monoclonal Rat IgG2A Clone # 139712
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant mouse ADAM10
Thr215-Glu673
Accession # O35598
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Phycoerythrin (Excitation= 488 nm, Emission= 565-605 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25-1 µg/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of ADAM10 antibody in Mouse Splenocytes antibody by Flow Cytometry. View Larger

Detection of ADAM10 in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with Rat Anti-Mouse ADAM10 Ectodomain PE-conjugated Monoclonal Antibody (Catalog # FAB946P, filled histogram) or isotype control antibody (Catalog # IC006P, open histogram). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: ADAM10

ADAM10 (also known as Kuzbanian, mammalian disintegrin metalloprotease, myelin-associated metalloproteinase) is a member of the ADAM family that contains a disintegrin and metalloprotease-like domain (1, 2). Like other membrane-anchored ADAMs, ADAM10 consists of the following domains, pro with a cysteine switch and furin cleavage sequence, catalytic with the zinc-binding site and Met-turn expected for reprolysins, disintegrin-like, cysteine-rich, EGF-like, transmembrane, and cytoplasmic. ADAM10 is highly conserved, with 97% amino acid identity between mouse, rat, bovine and human and 45% identity between mouse and Drosophila. The active enzyme processes notch, notch ligand delta, and amyloid protein precursor at the alpha site, playing an important role in neurogenesis (3, 4). It also processes the 26 kDa membrane-anchored pro-tumor necrosis factor-alpha (TNF-alpha ) to the 17 kDa mature TNF-alpha (5). It cleaves myelin basic protein and type IV collagen (6, 7). ADAM10 is widely expressed in tissues and resides both on the cell surface and in the cell (8, 9).

References
  1. Rooke, J. et al. (1996) Science 273:1227.
  2. Pan, D. and Rubin, G.M. (1997) Cell 90:271.
  3. Qi, H. et al. (1999) Science 283:91.
  4. Lammich, S. et al. (1999) Proc. Natl. Acad. Sci. USA 96:3922.
  5. Rosendahl, M.S. et al. (1997) J. Biol. Chem. 272:24588.
  6. Chantry, A. et al. (1989) J. Biol. Chem. 264:21603.
  7. Millichip, M.I. et al. (1998) Biochem. Biophys. Res. Comm. 245:594.
  8. Chantry, A. and Glynn, P. (1990) Biochem. J. 268:245.
  9. Fahrenholz, F.S. et al. (2000) Ann. N.Y. Acad. Sci. 920:215.
Long Name
A Disintegrin and Metalloprotease-like Domain 10
Entrez Gene IDs
102 (Human); 11487 (Mouse)
Alternate Names
a disintegrin and metalloprotease domain 10; a disintegrin and metalloproteinase domain 10; AD10; ADAM 10; ADAM metallopeptidase domain 10; ADAM10; CD156c antigen; CD156c; CDw156; disintegrin and metalloproteinase domain-containing protein 10; EC 3.4.24; HsT18717; kuz; Kuzbanian protein homolog; Kuzbanian; MADM; MADMEC 3.4.24.81; Mammalian disintegrin-metalloprotease

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Citations for Mouse ADAM10 Ectodomain PE-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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  1. Cellular Plasticity of Defa4Cre-Expressing Paneth Cells in Response to Notch Activation and Intestinal Injury
    Authors: JC Jones, CD Brindley, NH Elder, MG Myers, MW Rajala, CM Dekaney, EN McNamee, MR Frey, NF Shroyer, PJ Dempsey
    Cell Mol Gastroenterol Hepatol, 2018-11-27;7(3):533-554.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC, IHC-F
  2. Crucial role of the Rap G protein signal in Notch activation and leukemogenicity of T-cell acute lymphoblastic leukemia.
    Authors: Doi, Keiko, Imai, Takahiko, Kressler, Christop, Yagita, Hideo, Agata, Yasutosh, Vooijs, Marc, Hamazaki, Yoko, Inoue, Joe, Minato, Nagahiro
    Sci Rep, 2015-01-23;5(0):7978.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. Adrenergic regulation of IgE involves modulation of CD23 and ADAM10 expression on exosomes.
    Authors: Padro C, Shawler T, Gormley M, Sanders V
    J Immunol, 2013-10-18;191(11):5383-97.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  4. ADAM17 is regulated by a rapid and reversible mechanism that controls access to its catalytic site.
    Authors: Le Gall SM, Maretzky T, Issuree PD, Niu XD, Reiss K, Saftig P, Khokha R, Lundell D, Blobel CP
    J. Cell. Sci., 2010-10-27;123(0):3913-22.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  5. Identification of ADAM10 as a major TNF sheddase in ADAM17-deficient fibroblasts.
    Authors: Mezyk-Kopec R, Bzowska M, Stalinska K, Chelmicki T, Podkalicki M, Jucha J, Kowalczyk K, Mak P, Bereta J
    Cytokine, 2009-04-05;46(3):309-15.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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