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Mouse ADAM15 Ectodomain Antibody

Catalog #: AF945
2 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF945
AF945-SP
Product Details
Citations (2)
FAQs
Supplemental Products
Reviews
Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse ADAM15 Ectodomain Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse ADAM15 Ectodomain in direct ELISAs and Western blots. In Western blots (under reducing and non-reducing conditions), no cross-reactivity with the ectodomains of recombinant mouse (rm) ADAM9, rmADAM10, recombinant human (rh) ADAM8, rhADAM15, and rhADAM17 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant mouse ADAM15
Arg18-Ser694
Accession # O88839
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse ADAM15
Immunohistochemistry
5-15 µg/mL
Perfusion fixed frozen sections of mouse brain (cerebellum)
Immunoprecipitation
25 µg/mL
Conditioned cell culture medium spiked with Recombinant Mouse ADAM15, see our available Western blot detection antibodies

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ADAM15

ADAM15, also known as MDC15 or metargidin, is a widely expressed metalloproteinase. It is a type I membrane protein that may be involved in cell adhesion and shedding of cell surface proteins.

Long Name
A Disintegrin and Metalloprotease-like Domain 15
Entrez Gene IDs
8751 (Human); 11490 (Mouse)
Alternate Names
ADAM 15; ADAM metallopeptidase domain 15; ADAM15; disintegrin-like, and cysteine-rich protein 15; EC 3.4.24; MDC15; MDC-15; Metargidin

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Citations for Mouse ADAM15 Ectodomain Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. An Adam15 amplification loop promotes vascular endothelial growth factor-induced ocular neovascularization.
    Authors: Xie B, Shen J, Dong A, Swaim M, Hackett SF, Wyder L, Worpenberg S, Barbieri S, Campochiaro PA
    FASEB J., 2008-04-01;22(8):2775-83.
    Species: Mouse
    Sample Types: Tissue Homogenates, Whole Tissue
    Applications: IHC-Fr, Immunoprecipitation
  2. Coregulation of vascular tube stabilization by endothelial cell TIMP-2 and pericyte TIMP-3.
    Authors: Saunders WB, Bohnsack BL, Faske JB, Anthis NJ, Bayless KJ, Hirschi KK, Davis GE
    J. Cell Biol., 2006-10-09;175(1):179-91.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot

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