Mouse ADAM33 Ectodomain Antibody

Catalog # Availability Size / Price Qty
MAB2434
MAB2434-SP
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Mouse ADAM33 Ectodomain Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse ADAM33 Ectodomain in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant mouse ADAM9, 10, 15, recombinant human (rh) ADAM8, rhTACE/ADAM17, rhADAM19, or rhADAM33 is observed.
Source
Monoclonal Rat IgG2B Clone # 219515
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse ADAM33 Ectodomain
Glu205-Ala702
Accession # Q923W9
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Mouse ADAM33 Ectodomain under non-reducing conditions only
Immunoprecipitation
25 µg/mL
Conditioned cell culture medium spiked with Recombinant Mouse ADAM33, see our available Western blot detection antibodies

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ADAM33

ADAM33 is a transmembrane protein that is widely expressed in mouse tissues. Human ADAM33 has been associated with asthma and bronchial hyperresponsiveness.

Long Name
A Disintegrin and Metalloproteinase Domain 33
Entrez Gene IDs
80332 (Human); 110751 (Mouse)
Alternate Names
a disintegrin and metalloprotease 33; a disintegrin and metalloproteinase domain 33; ADAM 33; ADAM metallopeptidase domain 33; ADAM33; C20orf153; chromosome 20 open reading frame 153; disintegrin and metalloproteinase domain-containing protein 33; disintegrin and reprolysin metalloproteinase family protein; dJ964F7.1; DKFZp434K0521; EC 3.4.24.-; FLJ35308; FLJ36751; MGC149823; MGC71889

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