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Mouse Adiponectin/Acrp30 Antibody

Catalog #: MAB1119
5 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB1119-SP
MAB1119-100
MAB1119-500
Detection of Mouse Adiponectin/Acrp30 by Western Blot.
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Product Details
Citations (5)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse Adiponectin/Acrp30 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects full-length mouse Adiponectin (aa 18‑247) in direct ELISAs and Western blots. Shows no cross-reactivity with recombinant human Adiponectin. Does not detect the C-terminal globular domain of mouse Adiponectin (aa 111‑247).
Source
Monoclonal Rat IgG2A Clone # 173205
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Adiponectin
Glu18-Asn247
Accession # Q60994
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
2 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Mouse Adiponectin/Acrp30 antibody by Western Blot. View Larger

Detection of Mouse Adiponectin/Acrp30 by Western Blot. Western blot shows lysates of mouse adipose tissue. PVDF membrane was probed with 2 µg/mL of Mouse Adiponectin/Acrp30 Monoclonal Antibody (Catalog # MAB1119) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Adiponectin/Acrp30 at approximately 34 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Adiponectin/Acrp30

Adiponectin, also known as Acrp30, is an adipocyte-derived protein with wide ranging paracrine and endocrine effects on metabolism and inflammation. It promotes adipocyte differentiation, fatty acid catabolism, and insulin sensitivity, and is negatively correlated with obesity, type 2 diabetes, and atherogenesis. In this context, adiponectin is an anti-inflammatory agent, but it exerts pro-inflammatory effects in nonmetabolic disorders such as rheumatoid arthritis and inflammatory bowel disease (1 - 3). Adiponectin interacts with the receptors AdipoR1 and AdipoR2, calreticulin, and Cadherin-13/T-Cadherin, as well as with several growth factors (4 - 7). Mature mouse adiponectin consists of a 66 amino acid (aa) N-terminal collagenous region and a 137 aa C-terminal C1q-like globular domain which can be cleaved by a leukocyte-derived elastase (8 - 10). Mature mouse adiponectin shares 83% and 91% amino acid (aa) sequence identity with human and rat adiponectin, respectively. Adiponectin associates into trimers that may assemble into medium molecular weight (MMW) hexamers and then into >300 kDa high molecular weight (HMW) oligomers (11 - 13). The glycosylation of four hydroxylated lysine residues in the collagenous domain is required for the intracellular formation of HMW complexes (14). The various multimeric forms of adiponectin exhibit distinct tissue specific and gender specific profiles and activities (13, 15).

References
  1. Lara-Castro, C. et al. (2007) Curr. Opin. Lipidol. 18:263.
  2. Tilg, H. and A.R. Moschen (2006) Nat. Rev. Immunol. 6:772.
  3. Fantuzzi, G. (2008) J. Allergy Clin. Immunol. 121:326.
  4. Yamauchi, T. et al. (2007) Nat. Med. 13:332.
  5. Takemura, Y. et al. (2007) J. Clin. Invest. 117:375.
  6. Hug, C. et al. (2004) Proc. Natl. Acad. Sci. 101:10308.
  7. Wang, Y. et al. (2005) J. Biol. Chem. 280:18341.
  8. Scherer, P.E. et al. (1995) J. Biol. Chem. 270:26746.
  9. Hu, E. et al. (1996) J. Biol. Chem. 271:10697.
  10. Waki, H. et al. (2005) Endocrinology 146:790.
  11. Waki, H. et al. (2003) J. Biol. Chem. 278:40352.
  12. Tsao, T.S. et al. (2003) J. Biol. Chem. 278:50810.
  13. Wang, Y. et al. (2008) Biochem. J. 409:623.
  14. Wang, H. et al. (2006) J. Biol. Chem. 281:16391.
  15. Pajvani, U.B. et al. (2003) J. Biol. Chem. 278:9073.
Entrez Gene IDs
9370 (Human); 11450 (Mouse); 246253 (Rat)
Alternate Names
ACDC; Acrp30; ACRP30ADPN; adipocyte, C1Q and collagen domain containing; Adiponectin; adiponectin, C1Q and collagen domain containing; AdipoQ; ADIPQTL1; ApM1; apM-1; APM1APM-1; C1q and collagen domain-containing protein; GBP28; GBP28apM1; Gelatin-binding protein

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Citations for Mouse Adiponectin/Acrp30 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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  1. Loss of RUBCN/rubicon in adipocytes mediates the upregulation of autophagy to promote the fasting response
    Authors: T Yamamuro, S Nakamura, K Yanagawa, A Tokumura, T Kawabata, A Fukuhara, H Teranishi, M Hamasaki, I Shimomura, T Yoshimori
    Autophagy, 2022-03-14;0(0):1-11.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Immunocytochemistry
  2. Age-dependent loss of adipose Rubicon promotes metabolic disorders via excess autophagy
    Authors: T Yamamuro, T Kawabata, A Fukuhara, S Saita, S Nakamura, H Takeshita, M Fujiwara, Y Enokidani, G Yoshida, K Tabata, M Hamasaki, A Kuma, K Yamamoto, I Shimomura, T Yoshimori
    Nat Commun, 2020-08-18;11(1):4150.
    Species: Mouse
    Sample Types: Protein, Tissue Homogenates
    Applications: Western Blot
  3. The sex specific effect of alcohol consumption on circulating levels of CTRP3
    Authors: Ashley R. DeGroat, Christina K. Fleming, Samantha M. Dunlay, Kendra L. Hagood, Jonathan P. Moorman, Jonathan M. Peterson
    PLOS ONE
  4. Metabolic profiling of follistatin overexpression: a novel therapeutic strategy for metabolic diseases
    Authors: R Singh, S Pervin, SJ Lee, A Kuo, V Grijalva, J David, L Vergnes, ST Reddy
    Diabetes Metab Syndr Obes, 2018-03-26;11(0):65-84.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  5. Adiponectin reduces plasma triglyceride by increasing VLDL triglyceride catabolism.
    Authors: Qiao L, Zou C, van der Westhuyzen DR, Shao J
    Diabetes, 2008-03-28;57(7):1824-33.
    Species: Mouse
    Sample Types: Serum
    Applications: Western Blot

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