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Mouse Adiponectin/Acrp30 Antibody

Catalog #: AF1119
34 Citations 1 Review Datasheet / COA / SDS
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AF1119
AF1119-SP
Adiponectin/Acrp30 in ST-2 mouse bone marrow-derived stromal cell line differentiated to adipocytes.
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Citations (34)
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Reviews (1)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse Adiponectin/Acrp30 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse Adiponectin/Acrp30 in direct ELISAs and Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Adiponectin/Acrp30
Glu18-Asn247
Accession # Q60994
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse Adiponectin/Acrp30 (Catalog # 5095-AC)
Immunocytochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry Adiponectin/Acrp30 antibody in ST-2 mouse bone marrow-derived stromal cell line differentiated to adipocytes by Immunocytochemistry (ICC). View Larger

Adiponectin/Acrp30 in ST-2 mouse bone marrow-derived stromal cell line differentiated to adipocytes. Adiponectin/Acrp30 was detected in immersion fixed ST-2 mouse bone marrow-derived stromal cell line differentiated to adipocytes using Goat Anti-Mouse Adiponectin/Acrp30 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1119) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; NL001) and counter-stained with DAPI (blue). Specific staining was localized to cell surfaces and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Immunocytochemistry Adiponectin/Acrp30 antibody in human mesenchymal stem cells differentiated to adipocytes by Immunocytochemistry (ICC). View Larger

Adiponectin/Acrp30 in human mesenchymal stem cells differentiated to adipocytes. Adiponectin/Acrp30 was detected in immersion fixed human mesenchymal stem cells differentiated to adipocytes using Goat Anti-Mouse Adiponectin/Acrp30 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1119) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; NL001) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Western Blot Detection of Mouse Adiponectin/Acrp30 by Western Blot View Larger

Detection of Mouse Adiponectin/Acrp30 by Western Blot Chronic administration of compound 21 increases the abundance of adiponectin and UCP‐1 in adipose tissue. (A) and (C) Abundance of adiponectin and UCP‐1 in adipose tissue as determined by western blotting. (B) and (D) Levels of adiponectin and UCP‐1 as determined by immunohistochemistry. Quantification of specific bands was performed with Gel‐Pro Analyzer software. (A) and (C) Scatter dot plots show individual values and superimposed bar graphs represent the means ± S.E. (B) and (D) Positive adiponectin and UCP‐1 staining was quantified using Image‐Pro Plus 4.5 software. Data were analyzed by unpaired two‐tailed Student's t test, where **P < 0.01 when compared with saline‐treated control mice. For adiponectin, (C21, n = 9 per group; control, n = 9 per group). For UCP‐1, (C21, n = 9 per group, control, n = 10 per group). Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/30156060), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Adiponectin/Acrp30

Adiponectin, also known as Acrp30, is an adipocyte-derived protein with wide ranging paracrine and endocrine effects on metabolism and inflammation. It promotes adipocyte differentiation, fatty acid catabolism, and insulin sensitivity, and is negatively correlated with obesity, type 2 diabetes, and atherogenesis. In this context, adiponectin is an anti-inflammatory agent, but it exerts pro-inflammatory effects in nonmetabolic disorders such as rheumatoid arthritis and inflammatory bowel disease (1-3). Adiponectin interacts with the receptors AdipoR1 and AdipoR2, calreticulin, and Cadherin-13/T-Cadherin, as well as with several growth factors (4-7). Mature mouse adiponectin consists of a 66 amino acid (aa) N-terminal collagenous region and a 137 aa C-terminal C1q-like globular domain which can be cleaved by a leukocyte-derived elastase (8-10). Mature mouse adiponectin shares 83% and 91% amino acid (aa) sequence identity with human and rat adiponectin, respectively. Adiponectin associates into trimers that may assemble into medium molecular weight (MMW) hexamers and then into >300 kDa high molecular weight (HMW) oligomers (11-13). The glycosylation of four hydroxylated lysine residues in the collagenous domain is required for the intracellular formation of HMW complexes (14). The various multimeric forms of adiponectin exhibit distinct tissue specific and gender specific profiles and activities (13, 15).

References
  1. Lara-Castro, C. et al. (2007) Curr. Opin. Lipidol. 18:263.
  2. Tilg, H. and A.R. Moschen (2006) Nat. Rev. Immunol. 6:772.
  3. Fantuzzi, G. (2008) J. Allergy Clin. Immunol. 121:326.
  4. Yamauchi, T. et al. (2007) Nat. Med. 13:332.
  5. Takemura, Y. et al. (2007) J. Clin. Invest. 117:375.
  6. Hug, C. et al. (2004) Proc. Natl. Acad. Sci. USA 101:10308.
  7. Wang, Y. et al. (2005) J. Biol. Chem. 280:18341.
  8. Scherer, P.E. et al. (1995) J. Biol. Chem. 270:26746.
  9. Hu, E. et al. (1996) J. Biol. Chem. 271:10697.
  10. Waki, H. et al. (2005) Endocrinology 146:790.
  11. Waki, H. et al. (2003) J. Biol. Chem. 278:40352.
  12. Tsao, T.S. et al. (2003) J. Biol. Chem. 278:50810.
  13. Wang, Y. et al. (2008) Biochem. J. 409:623.
  14. Wang, H. et al. (2006) J. Biol. Chem. 281:16391.
  15. Pajvani, U.B. et al. (2003) J. Biol. Chem. 278:9073.
Entrez Gene IDs
9370 (Human); 11450 (Mouse); 246253 (Rat)
Alternate Names
ACDC; Acrp30; ACRP30ADPN; adipocyte, C1Q and collagen domain containing; Adiponectin; adiponectin, C1Q and collagen domain containing; AdipoQ; ADIPQTL1; ApM1; apM-1; APM1APM-1; C1q and collagen domain-containing protein; GBP28; GBP28apM1; Gelatin-binding protein

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Citations for Mouse Adiponectin/Acrp30 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

34 Citations: Showing 1 - 10
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  1. ER stress decreases exosome production through adiponectin/T-cadherin-dependent and -independent pathways
    Authors: Keita Fukuoka, Ryohei Mineo, Shunbun Kita, Shiro Fukuda, Tomonori Okita, Emi Kawada-Horitani et al.
    Journal of Biological Chemistry
  2. Succination of Protein Disulfide Isomerase Links Mitochondrial Stress to Endoplasmic Reticulum Stress in the Adipocyte during Diabetes
    Authors: AM Manuel, MD Walla, A Faccenda, SL Martin, RM Tanis, GG Piroli, J Adam, B Kantor, B Mutus, DM Townsend, N Frizzell
    Antioxid. Redox Signal., 2017-05-19;0(0):.
  3. Regulation Of Coronary Endothelial Function By Interactions Between TNF-Alpha, LOX-1 And Adiponectin In Apolipoprotein E Knock Out Mice
    Authors: Xiuping Chen, Hanrui Zhang, Michael A. Hill, Cuihua Zhang, Yoonjung Park
    Journal of Vascular Research
  4. Secreted frizzled‑related protein 4 promotes brown adipocyte differentiation
    Authors: Hua Guan, Huiyuan Zheng, Jin Zhang, Aoqi Xiang, Yongxin Li, Huadong Zheng et al.
    Experimental and Therapeutic Medicine
  5. Pharmacological HIF-1 activation upregulates extracellular vesicle production synergistically with adiponectin through transcriptional induction and protein stabilization of T-cadherin
    Authors: Fujii, K;Fujishima, Y;Kita, S;Kawada, K;Fukuoka, K;Sakaue, TA;Okita, T;Kawada-Horitani, E;Nagao, H;Fukuda, S;Maeda, N;Nishizawa, H;Shimomura, I;
    Scientific reports
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  6. Isthmin-1 attenuates allergic Asthma by stimulating adiponectin expression and alveolar macrophage efferocytosis in mice
    Authors: Tee, JH;Vijayakumar, U;Shanmugasundaram, M;Lam, TYW;Liao, W;Yang, Y;Wong, WSF;Ge, R;
    Respiratory research
    Species: Human, Mouse, Transgenic Mouse
    Sample Types: Cell Culture Supernates, Tissue Homogenates
    Applications: Western Blot
  7. Role of Adiponectin-Notch pathway in cognitive dysfunction associated with depression and in the therapeutic effect of physical exercise
    Authors: J You, L Sun, J Wang, F Sun, W Wang, D Wang, X Fan, D Liu, Z Xu, C Qiu, J Chen, H Yan, B Liu
    Aging Cell, 2021-05-30;0(0):e13387.
    Species: Mouse
    Sample Types: Serum
    Applications: Western Blot
  8. Adiponectin Stimulates Exosome Release to Enhance Mesenchymal Stem-Cell-Driven Therapy of Heart Failure in Mice
    Authors: Y Nakamura, S Kita, Y Tanaka, S Fukuda, Y Obata, T Okita, H Nishida, Y Takahashi, Y Kawachi, Y Tsugawa-Sh, Y Fujishima, H Nishizawa, Y Takakura, S Miyagawa, Y Sawa, N Maeda, I Shimomura
    Mol. Ther., 2020-07-10;0(0):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  9. Chronic administration of the angiotensin type 2 receptor agonist C21 improves insulin sensitivity in C57BL/6 mice
    Authors: DT Quiroga, MC Muñoz, C Gil, M Pffeifer, JE Toblli, UM Steckeling, JF Giani, FP Dominici
    Physiol Rep, 2018-08-01;6(16):e13824.
    Species: Mouse
    Sample Types: Tissue Homogenates, Whole Tissue
    Applications: IHC, Western Blot
  10. Adiponectin modulates ventral tegmental area dopamine neuron activity and anxiety-related behavior through AdipoR1
    Authors: F Sun, Y Lei, J You, C Li, L Sun, J Garza, D Zhang, M Guo, PE Scherer, D Lodge, XY Lu
    Mol. Psychiatry, 2018-07-09;0(0):.
    Species: Mouse
    Sample Types: Plasma
    Applications: Western Blot
  11. Adiponectin/T-cadherin system enhances exosome biogenesis and decreases cellular ceramides by exosomal release
    Authors: Y Obata, S Kita, Y Koyama, S Fukuda, H Takeda, M Takahashi, Y Fujishima, H Nagao, S Masuda, Y Tanaka, Y Nakamura, H Nishizawa, T Funahashi, B Ranscht, Y Izumi, T Bamba, E Fukusaki, R Hanayama, S Shimada, N Maeda, I Shimomura
    JCI Insight, 2018-04-19;3(8):.
    Species: Mouse
    Sample Types:
  12. SDF-1 is an Autocrine Insulin-Desensitizing Factor in Adipocytes
    Authors: J Shin, A Fukuhara, T Onodera, S Kita, C Yokoyama, M Otsuki, I Shimomura
    Diabetes, 2018-03-26;0(0):.
    Species: Mouse
    Sample Types: Cell Lysate
    Applications: Western Blot
  13. Retinol-binding protein 7 is an endothelium-specific PPAR? cofactor mediating an antioxidant response through adiponectin
    Authors: C Hu, HL Keen, KT Lu, X Liu, J Wu, DR Davis, SC Ibeawuchi, S Vogel, FW Quelle, CD Sigmund
    JCI Insight, 2017-03-23;2(6):e91738.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  14. The unique prodomain of T-cadherin plays a key role in adiponectin binding with the essential extracellular cadherin repeats 1 and 2
    Authors: S Fukuda, S Kita, Y Obata, Y Fujishima, H Nagao, S Masuda, Y Tanaka, H Nishizawa, T Funahashi, J Takagi, N Maeda, I Shimomura
    J. Biol. Chem, 2017-03-21;0(0):.
    Species: Mouse
    Sample Types: Serum
    Applications: Western Blot
  15. Role of the adipose PPAR?-adiponectin axis in susceptibility to stress and depression/anxiety-related behaviors
    Mol. Psychiatry, 2016-12-13;0(0):.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  16. Chronic blockade of the AT2 receptor with PD123319 impairs insulin signaling in C57BL/6 mice
    Authors: M C Muñoz
    Peptides, 2016-12-12;0(0):.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  17. Positive feedback regulation between adiponectin and T-cadherin impacts adiponectin levels in tissue and plasma of male mice.
    Authors: Matsuda, Keisuke, Fujishima, Yuya, Maeda, Norikazu, Mori, Takuya, Hirata, Ayumu, Sekimoto, Ryohei, Tsushima, Yu, Masuda, Shigeki, Yamaoka, Masaya, Inoue, Kana, Nishizawa, Hitoshi, Kita, Shunbun, Ranscht, Barbara, Funahashi, Tohru, Shimomura, Iichiro
    Endocrinology, 2014-12-16;156(3):934-46.
    Species: Mouse
    Sample Types: Tissue Homogenates, Whole Cells, Whole Tissue
    Applications: IHC, Western Blot
  18. Ultrastructural localization of adiponectin protein in vasculature of normal and atherosclerotic mice.
    Authors: Mori T, Koyama Y, Maeda N, Nakamura Y, Fujishima Y, Matsuda K, Funahashi T, Shimada S, Shimomura I
    Sci Rep, 2014-05-08;4(0):4895.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  19. Distinct fibroblast lineages determine dermal architecture in skin development and repair.
    Authors: Driskell R, Lichtenberger B, Hoste E, Kretzschmar K, Simons B, Charalambous M, Ferron S, Herault Y, Pavlovic G, Ferguson-Smith A, Watt F
    Nature, 2013-12-12;504(7479):277-81.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  20. Adiponectin promotes monocyte-to-fibroblast transition in renal fibrosis.
    Authors: Yang J, Lin S, Chen G, He L, Hu Z, Chan L, Trial J, Entman M, Wang Y
    J Am Soc Nephrol, 2013-07-05;24(10):1644-59.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  21. T-cadherin is essential for adiponectin-mediated revascularization.
    Authors: Parker-Duffen, Jennifer, Nakamura, Kazuto, Silver, Marcy, Kikuchi, Ryosuke, Tigges, Ulrich, Yoshida, Sumiko, Denzel, Martin S, Ranscht, Barbara, Walsh, Kenneth
    J Biol Chem, 2013-07-03;288(34):24886-97.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: Western Blot
  22. Leucine-rich glioma inactivated 3 associates negatively with adiponectin.
    Authors: Kim H, Kwon N, Baek K, Kim D, Yun H
    Cytokine, 2013-03-30;62(2):206-9.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  23. Vascular smooth muscle cell-derived adiponectin: a paracrine regulator of contractile phenotype.
    Authors: Ding M, Carrao A, Wagner R, Xie Y, Jin Y, Rzucidlo E, Yu J, Li W, Tellides G, Hwa J, Aprahamian T, Martin K
    J Mol Cell Cardiol, 2011-09-17;52(2):474-84.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  24. Molecular, biochemical and functional characterizations of C1q/TNF family members: adipose-tissue-selective expression patterns, regulation by PPAR-gamma agonist, cysteine-mediated oligomerizations, combinatorial associations and metabolic functions.
    Authors: Wong GW, Krawczyk SA, Kitidis-Mitrokostas C, Revett T, Gimeno R, Lodish HF
    Biochem. J., 2008-12-01;416(2):161-77.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Western Blot
  25. Adiponectin modulates inflammatory reactions via calreticulin receptor-dependent clearance of early apoptotic bodies.
    Authors: Takemura Y, Ouchi N, Shibata R, Aprahamian T, Kirber MT, Summer RS, Kihara S, Walsh K
    J. Clin. Invest., 2007-01-25;117(2):375-86.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  26. Adiponectin alters renal calcium and phosphate excretion through regulation of klotho expression
    Authors: Joseph M. Rutkowski, Johanne Pastor, Kai Sun, S. Claire Park, I. Alexandru Bobulescu, Christopher T. Chen et al.
    Kidney International
  27. Effects of Enriched Environment on COX-2, Leptin and Eicosanoids in a Mouse Model of Breast Cancer
    Authors: Rachida Nachat-Kappes, Alexandre Pinel, Kristell Combe, Bruno Lamas, Marie-Chantal Farges, Adrien Rossary et al.
    PLoS ONE
  28. Mice lacking angiotensin type 2 receptor exhibit a sex-specific attenuation of insulin sensitivity
    Authors: Diego T. Quiroga, Johanna G. Miquet, Lorena Gonzalez, Ana I. Sotelo, Marina C. Muñoz, Pedro M. Geraldes et al.
    Molecular and Cellular Endocrinology
  29. Soluble T-cadherin promotes pancreatic beta -cell proliferation by upregulating Notch signaling
    Authors: Tomonori Okita, Shunbun Kita, Shiro Fukuda, Keita Fukuoka, Emi Kawada-Horitani, Masahito Iioka et al.
    iScience
  30. Adiponectin Limits IFN-gamma and IL-17 Producing CD4 T Cells in Obesity by Restraining Cell Intrinsic Glycolysis
    Authors: Jayagopi Surendar, Stefan J. Frohberger, Indulekha Karunakaran, Vanessa Schmitt, Wiebke Stamminger, Anna-Lena Neumann et al.
    Frontiers in Immunology
  31. Adiponectin and its receptors are involved in hypertensive vascular injury
    Authors: R Guo, M Han, J Song, J Liu, Y Sun
    Mol Med Rep, 2017-10-25;0(0):.
  32. Native adiponectin in serum binds to mammalian cells expressing T-cadherin, but not AdipoRs or calreticulin
    Authors: Shunbun Kita, Shiro Fukuda, Norikazu Maeda, Iichiro Shimomura
    eLife
  33. Fish Oil-Derived Long-Chain n-3 Polyunsaturated Fatty Acids Reduce Expression of M1-Associated Macrophage Markers in an ex vivo Adipose Tissue Culture Model, in Part through Adiponectin
    Authors: Anna A. De Boer, Jennifer M. Monk, Danyelle M. Liddle, Krista A. Power, David W. L. Ma, Lindsay E. Robinson
    Frontiers in Nutrition
  34. Negative regulation of adiponectin secretion by receptor interacting protein 140 (RIP140)
    Authors: Ping-Chih Ho, Li-Na Wei
    Cellular Signalling

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Mouse Adiponectin/Acrp30 Antibody
By Anonymous on 04/26/2017