Mouse ALCAM/CD166 Antibody

Catalog #: MAB1172
4 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB1172
MAB1172-SP

All ALCAM/CD166 Antibodies

Product Details

Citations (4)

Reviews

Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse ALCAM/CD166 Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse ALCAM/CD166 in Western blots and direct ELISAs. In Western blots, shows 100% cross-reactivity with recombinant human (rh) ALCAM and no cross-reactivity with rhBCAM, rhEpCAM, recombinant mouse (rm) MAdCAM-1, rhMCAM, rmNCAM-L1, rmOCAM, or rmTROP-2.

Source

Monoclonal Rat IgG_2A Clone # 200622

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse ALCAM/CD166
Trp28-Lys527
Accession # AAC06342

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

Recombinant Mouse ALCAM/CD166 Fc Chimera (Catalog # 1172-AL)

Flow Cytometry

2.5 µg/10⁶ cells

Mouse splenocytes treated with PHA

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ALCAM/CD166

ALCAM, activated leukocyte cell adhesion molecule, is a type I membrane glycoprotein and a member of the immunoglobulin supergene family. It is also known as CD166, MEMD, SC-1/DM-GRASP/BEN in the chicken, and KG-CAM in the rat. ALCAM is expressed on thymic epithelial cells, activated B and T cells, and monocytes. ALCAM can bind itself homotypically and is also capable of binding CD6, NgCAM, and other, as of yet, unidentified brain proteins. ALCAM/CD6 interaction may be involved in T cell development and T cell regulation. Additionally, ALCAM/CD6 and ALCAM/NgCAM interactions may play roles in the nervous system. ALCAM has also been observed to be upregulated on highly metastasizing melanoma cell lines and may play a role in tumor migration. ALCAM is a 583 amino acid (aa) protein consisting of a 27 aa signal peptide, a 500 aa extracellular domain, a 24 aa transmembrane domain and a 32 aa cytoplasmic domain. The extracellular domain of ALCAM contains 5 Ig-like domains of which the amino-terminal V1 domain is essential for ligand binding and ALCAM‑mediated cell aggregation (1‑4).

References

Bowen, M.A. et al. (1995) J. Exp. Med. 181:2213.
Aruffo, A. et al. (1997) Immunol. Today 18:498.
Degen, W.G. et al. (1998) Am. J. Pathol. 152:805.
Van Kempen, L. et al. (2001) J. Biol. Chem. 276:25783.

Long Name

Activated Leukocyte Cell Adhesion Molecule

Entrez Gene IDs

214 (Human); 11658 (Mouse); 101867493 (Cynomolgus Monkey)

Alternate Names

activated leucocyte cell adhesion molecule; activated leukocyte cell adhesion moleculeFLJ38514; ALCAM; CD166 antigen; CD166; MEMDMGC71733

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Mouse ALCAM/CD166 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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A novel molecular magnetic resonance imaging agent targeting activated leukocyte cell adhesion molecule as demonstrated in mouse brain metastasis models
Authors: Niloufar Zarghami, Manuel Sarmiento Soto, Francisco Perez-Balderas, Alexandre A Khrapitchev, Christina Simoglou Karali, Vanessa A Johanssen et al.
Journal of Cerebral Blood Flow & Metabolism
Cbln1 regulates axon growth and guidance in multiple neural regions
Authors: P Han, Y She, Z Yang, M Zhuang, Q Wang, X Luo, C Yin, J Zhu, SR Jaffrey, SJ Ji
PloS Biology, 2022-11-17;20(11):e3001853.
Species: Transgenic Mouse
Sample Types: Whole Tissue
Applications: IHC
Immunization with a mimotope of GD2 ganglioside induces CD8+ T cells that recognize cell adhesion molecules on tumor cells.
Authors: Wierzbicki A, Gil M, Ciesielski M, Fenstermaker RA, Kaneko Y, Rokita H, Lau JT, Kozbor D
J. Immunol., 2008-11-01;181(9):6644-53.
Species: Mouse
Sample Types: Recombinant Protein, Whole Cells
Applications: Flow Cytometry, Western Blot
A New Nano-sized Iron Oxide Particle with High Sensitivity for Cellular Magnetic Resonance Imaging
Authors: Chih-Lung Chen, Haosen Zhang, Qing Ye, Wen-Yuan Hsieh, T. Kevin Hitchens, Hsin-Hsin Shen et al.
Molecular Imaging and Biology