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Mouse ALCAM/CD166 PE-conjugated Antibody

Catalog #: FAB1172P
6 Citations Datasheet / COA / SDS
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FAB1172P
Detection of ALCAM/CD166 in Mouse Splenocytes by Flow Cytometry.
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Citations (6)
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Mouse ALCAM/CD166 PE-conjugated Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse ALCAM in direct ELISAs and Western blots. In direct ELISAs, approximately 40% cross-reactivity with recombinant human (rh) ALCAM is observed, and less than 10% cross-reactivity with rhBCAM, recombinant mouse (rm) OCAM, and rmMAdCAM‑1 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse ALCAM/CD166
Trp28-Lys527
Accession # AAC06342
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Phycoerythrin (Excitation= 488 nm, Emission= 565-605 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of ALCAM/CD166 antibody in Mouse Splenocytes antibody by Flow Cytometry. View Larger

Detection of ALCAM/CD166 in Mouse Splenocytes by Flow Cytometry. Mouse Splenocytes either (A) activated or (B) resting were stained with Goat Anti-Mouse ALCAM/CD166 PE-conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # FAB1172P) and Rat Anti-Mouse B220/CD45R APC-conjugated Monoclonal Antibody (Catalog # FAB1217A). Quadrant markers were set based on control antibody staining (Catalog # IC108P). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: ALCAM/CD166

ALCAM, activated leukocyte cell adhesion molecule, is a type I membrane glycoprotein and a member of the immunoglobulin supergene family. It is also known as CD166, MEMD, SC-1/DM-GRASP/BEN in the chicken, and KG-CAM in the rat. ALCAM is expressed on thymic epithelial cells, activated B and T cells, and monocytes. ALCAM can bind itself homotypically and is also capable of binding CD6, NgCAM, and other, as of yet, unidentified brain proteins. ALCAM/CD6 interaction may be involved in T cell development and T cell regulation. Additionally, ALCAM/CD6 and ALCAM/NgCAM interactions may play roles in the nervous system. ALCAM has also been observed to be upregulated on highly metastasizing melanoma cell lines and may play a role in tumor migration. ALCAM is a 583 amino acid (aa) protein consisting of a 27 aa signal peptide, a 500 aa extracellular domain, a 24 aa transmembrane domain, and a 32 aa cytoplasmic domain. The extracellular domain of ALCAM contains 5 Ig-like domains of which the amino-terminal V1 domain is essential for ligand binding and ALCAM-mediated cell aggregation (1-4).  The ECD of mouse ALCAM shares 97.5% aa sequence identity with rat ALCAM ECD.

References
  1. Bowen, M.A. et al. (1995) J. Exp. Med. 181:2213.
  2. Aruffo, A. et al. (1997) Immunol. Today 18:498.
  3. Degen, W.G. et al. (1998) Am. J. Pathol. 152:805.
  4. Van Kempen, L. et al. (2001) J. Biol. Chem. 276:25783.
Long Name
Activated Leukocyte Cell Adhesion Molecule
Entrez Gene IDs
214 (Human); 11658 (Mouse); 101867493 (Cynomolgus Monkey)
Alternate Names
activated leucocyte cell adhesion molecule; activated leukocyte cell adhesion moleculeFLJ38514; ALCAM; CD166 antigen; CD166; MEMDMGC71733

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Citations for Mouse ALCAM/CD166 PE-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
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  1. Tumor-initiating stem cell shapes its microenvironment into an immunosuppressive barrier and pro-tumorigenic niche
    Authors: X He, SE Smith, S Chen, H Li, D Wu, PI Meneses-Gi, Y Wang, M Hembree, K Yi, X Zhao, F Guo, JR Unruh, LE Maddera, Z Yu, A Scott, A Perera, Y Wang, C Zhao, K Bae, A Box, JS Haug, F Tao, D Hu, DM Hansen, P Qian, S Saha, D Dixon, S Anant, D Zhang, EH Lin, W Sun, LM Wiedemann, L Li
    Cell Reports, 2021-09-07;36(10):109674.
  2. Cell Adhesion Molecule CD166/ALCAM Functions Within the Crypt to Orchestrate Murine Intestinal Stem Cell Homeostasis
    Authors: Nicholas R. Smith, Paige S. Davies, Trevor G. Levin, Alexandra C. Gallagher, Douglas R. Keene, Sidharth K. Sengupta et al.
    Cellular and Molecular Gastroenterology and Hepatology
  3. Heterogeneity and dynamics of active Kras-induced dysplastic lineages from mouse corpus stomach
    Authors: J Min, PN Vega, AC Engevik, JA Williams, Q Yang, LM Patterson, AJ Simmons, RJ Bliton, JW Betts, KS Lau, ST Magness, JR Goldenring, E Choi
    Nat Commun, 2019-12-05;10(1):5549.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  4. Intestinal Enteroendocrine Lineage Cells Possess Homeostatic and Injury-Inducible Stem Cell Activity
    Authors: KS Yan, O Gevaert, GXY Zheng, B Anchang, CS Probert, KA Larkin, PS Davies, ZF Cheng, JS Kaddis, A Han, K Roelf, RI Calderon, E Cynn, X Hu, K Mandleywal, J Wilhelmy, SM Grimes, DC Corney, SC Boutet, JM Terry, P Belgrader, SB Ziraldo, TS Mikkelsen, F Wang, RJ von Furste, NR Smith, P Chandrakes, R May, MAS Chrissy, R Jain, CA Cartwright, JC Niland, YK Hong, J Carrington, DT Breault, J Epstein, CW Houchen, JP Lynch, MG Martin, SK Plevritis, C Curtis, HP Ji, L Li, SJ Henning, MH Wong, CJ Kuo
    Cell Stem Cell, 2017-07-06;21(1):78-90.e6.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  5. IRF4 controls the positioning of mature B cells in the lymphoid microenvironments by regulating NOTCH2 expression and activity.
    Authors: Simonetti, Giorgia, Carette, Amanda, Silva, Kathryn, Wang, Haowei, De Silva, Nilushi, Heise, Nicole, Siebel, Christia, Shlomchik, Mark J, Klein, Ulf
    J Exp Med, 2013-12-09;210(13):2887-902.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  6. Identification of a common mesenchymal stromal progenitor for the adult haematopoietic niche
    Authors: Xingbin Hu, Mayra Garcia, Lihong Weng, Xiaoman Jung, Jodi L. Murakami, Bijender Kumar et al.
    Nature Communications

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