Mouse Ameloblastin Antibody

Catalog # Availability Size / Price Qty
AF3026
AF3026-SP
Product Details
Citations (2)
FAQs
Supplemental Products
Reviews

Mouse Ameloblastin Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse Ameloblastin in direct ELISAs and Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Ameloblastin
Val27-Pro407
Accession # O55189
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse Ameloblastin

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Ameloblastin

Ameloblastin, also known as amelin and sheathlin, is a 66 kDa secreted tooth-specific glycoprotein. It is a major enamel matrix protein that promotes enamel formation in teeth. Ameloblastin is secreted primarily by secretory ameloblasts. It is a cell adhesion molecule required for amelogenesis. In addition to the isoform used as immunogen, a second variant with a 15 aa insertion between Q113 and P114 (AAH 87927) is known. The amino acid sequence of mature mouse ameloblastin is 96% and 67% identical to that of rat and human ameloblastin, respectively.

Entrez Gene IDs
258 (Human); 11698 (Mouse)
Alternate Names
AI1F; AMBN; Ameloblastin

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Citations for Mouse Ameloblastin Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Differentiation of an ameloblast-lineage cell line (ALC) is induced by Sonic hedgehog signaling.
    Authors: Takahashi S, Kawashima N, Sakamoto K, Nakata A, Kameda T, Sugiyama T, Katsube K, Suda H
    Biochem. Biophys. Res. Commun., 2006-12-18;353(2):405-11.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  2. Co-Immunoprecipitation Reveals Interactions Between Amelogenin and Ameloblastin via Their Self-Assembly Domains
    Authors: Rucha Arun Bapat, Jingtan Su, Janet Moradian-Oldak
    Frontiers in Physiology

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