Mouse Aminoacylase/ACY1 Antibody

Catalog #: MAB2700 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB2700
MAB2700-SP

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse Aminoacylase/ACY1 Antibody Summary

Species Reactivity

Mouse

Specificity

Detects human Aminoacylase/ACY1 in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant human ACY1 is observed.

Source

Monoclonal Rat IgG_2B Clone # 475730

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse Aminoacylase/ACY1
Met1-Ser408
Accession # NP_079647

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

Recombinant Mouse Aminoacylase/ACY1 (Catalog # 2700-ZN)

Immunoprecipitation

25 µg/mL

Cell lysates spiked with Recombinant Mouse Aminoacylase/ACY1 (Catalog # 2700-ZN), see our available Western blot detection antibodies

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Aminoacylase/ACY1

Aminoacylase 1 (ACY1) is an intracellular soluble homodimeric zinc-binding enzyme. It is involved in the breakdown of acetylated amino acids generated during protein degradation by catalyzing the formation of free aliphatic amino acids from N-acetylated precursors. This enzyme is evolutionarily conserved between mouse and human. Mutations in the human ACY1 gene might cause inborn metabolic disorders. The amino acid sequence of mouse ACY1 is 93% and 85% identical to that of rat and human/chimpanzee.

Entrez Gene IDs

95 (Human); 109652 (Mouse)

Alternate Names

ACY1; ACY-1; ACY1D; acylase; aminoacylase 1; Aminoacylase; aminoacylase-1; EC 3.5.1; EC 3.5.1.14; N-acyl-L-amino-acid amidohydrolase