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Mouse Aminoacylase/ACY1 Antibody

Catalog #: AF2700
2 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF2700
AF2700-SP
Product Details
Citations (2)
FAQs
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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse Aminoacylase/ACY1 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse Aminoacylase/ACY1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 50% cross‑reactivity with recombinant human Aminoacylase/ACY1 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Aminoacylase/ACY1
Met1-Ser408
Accession # NP_079647
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse Aminoacylase/ACY1 (Catalog # 2700-ZN)
Immunoprecipitation
25 µg/mL
Conditioned cell culture medium spiked with Recombinant Mouse Aminoacylase/ACY1 (Catalog # 2700-ZN), see our available Western blot detection antibodies

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Aminoacylase/ACY1

The mouse ACY1 gene encodes aminoacylase, a member of the M20 family of metalloproteases (1). ACY1 plays a general role in the cytosolic breakdown of acetylated amino acids generated during protein degradation. It also interacts with sphingosine kinase type 1, which is involved in promoting cell growth and inhibiting apoptosis of tumor cells (2). The full-length protein is expressed and the purified enzyme is active in the assay.

References
  1. Lindner, H.A. et al. (2003) J. Biol. Chem. 278:44496.
  2. Maceyka, M. et al. (2004) FEBS Lett. 568:30.
Entrez Gene IDs
95 (Human); 109652 (Mouse)
Alternate Names
ACY1; ACY-1; ACY1D; acylase; aminoacylase 1; Aminoacylase; aminoacylase-1; EC 3.5.1; EC 3.5.1.14; N-acyl-L-amino-acid amidohydrolase

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Citations for Mouse Aminoacylase/ACY1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. A novel co-target of ACY1 governing plasma membrane translocation of SphK1 contributes to inflammatory and neuropathic pain
    Authors: Baowen Liu, Wenyao Wu, LingLing Cui, Xuemei Zheng, Ningbo Li, Xianwei Zhang et al.
    iScience
  2. A collagen IV-derived peptide disrupts ?5?1 integrin and potentiates Ang2/Tie2 signaling
    Authors: AC Mirando, J Shen, RLE Silva, Z Chu, NC Sass, VE Lorenc, JJ Green, PA Campochiar, AS Popel, NB Pandey
    JCI Insight, 2019-02-21;4(4):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC

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