Mouse Aminopeptidase A/ENPEP Antibody

Catalog #: AF2809
1 Citation Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF2809
AF2809-SP

Product Details

Citations (1)

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse Aminopeptidase A/ENPEP Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse Aminopeptidase A/ENPEP in direct ELISAs and Western blots. In Western blots, approximately 10% cross-reactivity with recombinant human ENPEP is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse Aminopeptidase A/ENPEP
Arg41-Pro945
Accession # P16406

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Mouse Aminopeptidase A/ENPEP

Immunoprecipitation

25 µg/mL

Conditioned cell culture medium spiked with Recombinant Mouse Aminopeptidase A/ENPEP, see our available Western blot detection antibodies

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Aminopeptidase A/ENPEP

The mouse ENPEP gene encodes Aminopeptidase A (APA), which is also known as glutamyl aminopeptidase (EAP), aspartate aminopeptidase, angiotensinase A, Ca²⁺-activated glutamate aminopeptidase, membrane aminopeptidase II and the BP-1/6C3 antigen (2). The various names reflect its substrate specificity (prefer Glu or Asp and angiotensin II as one of the physiological substrates), Ca²⁺-dependence, cellular location (type II membrane protein) and immunological reactivity to antibody BP-1/6C3. The deduced amino acid sequence of mouse ENPEP consists of a short cytoplasmic tail (residues 1 to 18), a transmembrane region (residue 19 to 39), and a long ectodomain (residues 40 to 945) (1). In addition to the N-terminal zinc metalloprotease domain, the ectodomain also contains the C‑terminal region, which functions as an intramolecular chaperone required for the correct folding, cell surface expression and activity (3). The purified rmENPEP consists of the ectodomain and is active in the assay described above.

References

Wu, Q. et al. (1990) Proc. Natl. Acad. Sci. USA 87:993.
Wang, J. and M.D. Cooper (2004) in Handbook of Proteolytic Enzymes (ed. Barrett, et al.) pp. 299, Academic Press, San Diego.
Rozenfeld, R. et al. (2004) J. Biol. Chem. 279:43285.

Entrez Gene IDs

2028 (Human); 13809 (Mouse)

Alternate Names

Aminopeptidase A; APA; AP-A; CD249 antigen; CD249; Differentiation antigen gp160; EAP; EC 3.4.11; EC 3.4.11.7; ENPEP; glutamyl aminopeptidase (aminopeptidase A); glutamyl aminopeptidase; gp160

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Product Datasheet

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Related Research Areas

Citation for Mouse Aminopeptidase A/ENPEP Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1