Mouse Aminopeptidase N/CD13 Antibody

Catalog #: MAB2335 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB2335
MAB2335-SP

Detection of Mouse Aminopeptidase N/CD13 by Western Blot.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse Aminopeptidase N/CD13 Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse Aminopeptidase in direct ELISAs and Western blots.

Source

Monoclonal Rat IgG_2A Clone # 945033

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

NS0 mouse myeloma cell line transfected with mouse Aminopeptidase
Lys69-Ser966
Accession # P97449

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Simple Western

10 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Mouse Aminopeptidase N/CD13 by Western Blot. Western blot shows lysates of mouse kidney tissue and mouse uterus tissue. PVDF membrane was probed with 1 µg/mL of Rat Anti-Mouse Aminopeptidase N/CD13 Monoclonal Antibody (Catalog # MAB2335) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Aminopeptidase N/CD13 at approximately 150 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Simple Western

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Detection of Mouse Aminopeptidase N/CD13 by Simple Western^TM. Simple Western lane view shows lysates of mouse kidney tissue, loaded at 0.2 mg/mL. A specific band was detected for Aminopeptidase N/CD13 at approximately 204 kDa (as indicated) using 10 µg/mL of Rat Anti-Mouse Aminopeptidase N/CD13 Monoclonal Antibody (Catalog # MAB2335) followed by 1:50 dilution of HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Aminopeptidase N/CD13

The mouse Anpep gene encodes Aminopeptidase N (APN), which is also known as microsomal aminopeptiase, alanyl aminopeptidase, Aminopeptidase M, CD13, or membrane protein p161 (1‑3). The deduced amino acid sequence of mouse APN consists of a short cytoplasmic tail (residues 2 to 8), a transmembrane region (residue 9 to 32), a Ser/Thr rich region and a zinc metalloprotease domain (residues 69 to 966). Widely expressed in many cells, tissues and species, APN cleaves the N-terminal amino acids from bioactive peptides, leading to their inactivation or degradation. The roles of APN in many fields, such as neuroscience, hematopoeitic cells, immune system, angiogenesis, cancer and viral infection, have been reviewed (4).

References

Chen, H. et al. (1996) J. Immunol. 157:2593.
Larsen, S.L. et al. (1996) J. Exp. Med. 184:183.
Hansen, A.S. et al. (1993) Eur. J. Immunol. 23:2358.
Turner, A.J. (2004) in Handbook of Proteolytic Enzymes (ed. Barrett, et al.) p. 289 Academic Press, San Diego.

Entrez Gene IDs

290 (Human); 16790 (Mouse); 397520 (Porcine); 102115663 (Cynomolgus Monkey)

Alternate Names

alanyl (membrane) aminopeptidase; Alanyl aminopeptidase; Aminopeptidase M; Aminopeptidase N; ANPEP; AP-M; APN; AP-N; CD13 antigen; CD13; CD13APN; EC 3.4.11; EC 3.4.11.2; gp150; LAP1; Microsomal aminopeptidase; Myeloid plasma membrane glycoprotein CD13; p150; PEPN; PEPNhAPN