Mouse Angiopoietin-like Protein 7/ANGPTL7 Antibody

Catalog #: AF4960 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF4960
AF4960-SP

Detection of Rat Angiopoietin-like Protein 7/ANGPTL7 by Western Blot.

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All Angiopoietin-like Protein 7/ANGPTL7 Antibodies

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Mouse Angiopoietin-like Protein 7/ANGPTL7 Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse Angiopoietin-like Protein 7/ANGPTL7 in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross-reactivity with recombinant human ANGPTL7 is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse Angiopoietin-like Protein 7/ANGPTL7
Gln22-Pro337
Accession # Q8R1Q3

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Rat Angiopoietin-like Protein 7/ANGPTL7 by Western Blot. Western blot shows lysates of rat eye tissue. PVDF Membrane was probed with 1 µg/mL of Mouse Angiopoietin-like Protein 7/ANGPTL7 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4960) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Angiopoietin-like Protein 7/ANGPTL7 at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Reconstitution Calculator

Preparation and Storage

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Angiopoietin-like Protein 7/ANGPTL7

Angiopoietin-like Protein 7 (ANGPTL7), also known as cornea-derived transcript 6 (CDT6), is a secreted 45 kDa protein that contains a coiled-coil domain (aa 30‑110) and a fibrinogen-like domain (aa 113‑334). ANGPTL7 is expressed in the stromal layer of the cornea, and it forms disulfide-linked homotetramers. It contributes to corneal morphogenesis by suppressing angiogenesis and promoting the deposition of extracellular matrix components. ANGPTL7 is up-regulated in glaucoma and accumulates in the aqueous humor. Mature mouse ANGPTL7 shares 88% and 98% sequence identity with human and rat ANGPTL7.

Long Name

Angiopoietin-like Protein 7

Entrez Gene IDs

10218 (Human); 654812 (Mouse); 102127960 (Cynomolgus Monkey); 714204 (Rhesus Macaque)

Alternate Names

angiopoietin-like 7; Angiopoietin-like factor; Angiopoietin-like Protein 7; angiopoietin-related protein 7; ANGPTL7; AngX; CDT6; CDT6angiopoietin-like factor (CDT6); Cornea-derived transcript 6 protein; dJ647M16.1; RP4-647M16.2