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Mouse B7-2/CD86 Antibody

Catalog #: AF-441-NA
3 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF-441-NA
AF-441-SP
IL‑2 Secretion Induced by CH‑1 Cell Surface B7-2 and Neutralization by Mouse B7‑2/CD86 Antibody.
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Citations (3)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse B7-2/CD86 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse B7‑2/CD86 in direct ELISAs and Western blots. In direct ELISAs and Western blots (non-reducing and reducing conditions), less than 15% cross-reactivity with recombinant human B7-2 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant mouse B7‑2/CD86
Extracellular domain
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse B7‑2/CD86 Fc Chimera (Catalog # 741-B2)
Neutralization
Measured by its ability to neutralize CH-1 cell surface B7-2-induced IL‑2 secretion in the Jurkat human acute T cell leukemia cell line. Linsley, P. et al. (1990) Proc. Natl. Acad. Sci. 87:5031. The Neutralization Dose (ND50) is typically 2-5 µg/mL in the presence of 10 µg/mL PHA.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization IL‑2 Secretion Induced by CH‑1 Cell Surface B7-2 and Neutralization by Mouse B7‑2/CD86 Antibody. View Larger

IL‑2 Secretion Induced by CH‑1 Cell Surface B7-2 and Neutralization by Mouse B7‑2/CD86 Antibody. The CH‑1 mouse B cell lymphoma cell line, which expresses B7‑1 and B7‑2 on the cell surface, co-stimulates IL‑2 secretion in the Jurkat human acute T cell leukemia cell line in the presence of PHA (10 µg/mL) in a dose-dependent manner (orange line), as measured by the Human IL‑2 Quantikine ELISA Kit (Catalog # D2050). Under these conditions, IL‑2 secretion elicited by CH‑1 cells is neutralized (green line) by increasing concentrations of Goat Anti-Mouse B7‑2/CD86 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-441-NA). The ND50 is typically 2-5 µg/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: B7-2/CD86

B7-1 and B7-2, together with their receptors CD28 and CTLA-4, constitute one of the dominant co‑stimulatory pathways that regulate T‑ and B‑cell responses. Although both CTLA-4 and CD28 can bind to the same ligands, CTLA-4 binds to B7-1 and B7‑2 with a 20‑100 fold higher affinity than CD28 and is involved in the down‑regulation of the immune response. B7-1 is expressed on activated B cells, activated T cells, and macrophages. B7‑2 is constitutively expressed on interdigitating dendritic cells, Langerhans cells, peripheral blood dendritic cells, memory B cells, and germinal center B cells. Additionally, B7-2 is expressed at low levels on monocytes and can be up‑regulated through interferon gamma. B7-1 and B7-2 are both members of the immunoglobulin superfamily. Mouse B7-2 is a 309 amino acid (aa) protein containing a putative 23 aa signal peptide, a 221 aa extracellular domain, a 21 aa transmembrane domain, and a 44 aa cytoplasmic domain. Mouse B7-2 and B7-1 share 28% amino acid identity. Mouse and human B7-2 share 50% amino acid identity. However, it has been observed that both human and mouse B7‑1 and B7‑2 can bind to either human or mouse CD28 and CTLA‑4, suggesting that there are conserved amino acids which form the B7-1/B7-2/CD28/CTLA-4 critical binding sites.

References
  1. Azuma, M. et al. (1993) Nature 366:76.
  2. Freeman, G.J. et al. (1993) Science 262:909.
  3. Freeman, G. et al. (1991) J. Exp. Med. 174:625.
  4. Selvakumar, A. et al. (1993) Immunogenetics 38:292.
  5. Chen, C. et al. (1994) J. Immunol. 152:4929.
  6. Freeman, G.J. et al. (1993) J. Exp. Med. 178:2185.
Entrez Gene IDs
942 (Human); 12524 (Mouse); 56822 (Rat); 102147235 (Cynomolgus Monkey)
Alternate Names
Activation B7-2 antigen; B70; B7-2 antigen; B72; B7-2; B-lymphocyte activation antigen B7-2; BU63; CD28 antigen ligand 2; CD28LG2B7-2 antigen); CD86 antigen; CD86 molecule; CD86; CTLA-4 counter-receptor B7.2; FUN-1; LAB72; MGC34413; T-lymphocyte activation antigen CD86

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Citations for Mouse B7-2/CD86 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. Dendritic cell derived exosomes loaded with immunoregulatory cargo reprogram local immune responses and inhibit degenerative bone disease in vivo
    Authors: Mahmoud Elashiry, Mohamed M. Elashiry, Ranya Elsayed, Mythily Rajendran, Carol Auersvald, Rana Zeitoun et al.
    Journal of Extracellular Vesicles
  2. Antigen-loaded ER microsomes from APC induce potent immune responses against viral infection.
    Authors: Sofra V, Mansour S, Liu M, Gao B, Primpidou E, Wang P, Li S
    Eur. J. Immunol., 2009-01-01;39(1):85-95.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  3. Suppression of activation and induction of apoptosis in RAW264.7 cells by amniotic membrane extract.
    Authors: He H, Li W, Chen SY, Zhang S, Chen YT, Hayashida Y, Zhu YT, Tseng SC
    Invest. Ophthalmol. Vis. Sci., 2008-07-09;49(10):4468-75.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC

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