Mouse B7-H2 Antibody

Catalog #: MAB10083 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB10083-100
MAB10083-SP

ICOS binding to Mouse B7-H2-transfected HEK293 Human Cell Line is Blocked by Mouse B7-H2 Antibody.

3 Images

All B7-H2 Antibodies

Product Details

Supplemental Products

Reviews

Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse B7-H2 Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse B7-H2 in direct ELISAs.

Source

Monoclonal Rat IgG_2A Clone # 987602

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse B7-H2
Met1-Lys279
Accession # Q9JHJ8

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Flow Cytometry

0.25 µg/10⁶ cells

See below

Blockade of Receptor-ligand Interaction

0.5 µg/10⁶ cells

See below

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Neutralization

Measured by its ability to neutralize B7-H2-induced IL-4 secretion in the D10.G4.1 mouse helper T cell line. The Neutralization Dose (ND₅₀) is typically 0.3-1.8 μg/mL in the presence of 2.5 ng/mL Recombinant Mouse B7-H2 and Rat Anti-Mouse CD3 Monoclonal Antibody.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Blockade of Receptor-ligand Interaction

View Larger

ICOS binding to Mouse B7-H2-transfected HEK293 Human Cell Line is Blocked by Mouse B7-H2 Antibody. In a functional flow cytometry test, Recombinant Mouse ICOS Fc Chimera (Catalog # 168-CS, 500 ng/mL) binds to HEK293 human embryonic kidney cell line transfected with recombinant mouse B7-H2 (black dotted line). Binding is completely blocked (orange histogram) by 0.5 µg/mL of Rat Anti-Mouse B7-H2 Monoclonal Antibody (Catalog # MAB10083). Rat IgG2A Isotype Control (Catalog # MAB006) at 0.5 µg/mL was used as a control (blue line). Ligand binding was detected by staining cells with Mouse Anti-Human IgG Fc APC-conjugated Monoclonal Antibody (Catalog # FAB110A).

Flow Cytometry

View Larger

Detection of Mouse B7-H2 in HEK293 Human Cell Line Transfected with Mouse B7-H2 and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with (A) mouse B7-H2 or (B) irrelevant transfectants and eGFP was stained with Rat Anti-Mouse B7-H2 Monoclonal Antibody (Catalog # MAB10083) followed by APC-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113). Quadrant markers were set based on control antibody staining (Catalog # MAB006). View our protocol for Staining Membrane-associated Proteins.

Neutralization

View Larger

IL-4 Secretion Induced by B7-H2 and Neutralization by Mouse B7-H2 Antibody. Recombinant Mouse B7-H2 (Catalog # 8127-B7) induces IL-4 secretion in the D10.G4.1 mouse helper T cell line in the presence of Rat Anti-Mouse CD3 Monoclonal Antibody (Catalog # MAB4841) in a dose-dependent manner (orange line), as measured by the Mouse IL-4 Quantikine ELISA Kit (Catalog # M4000B). Under these conditions, IL-4 secretion elicited by B7-H2 (2.5 µg/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse B7-H2 Monoclonal Antibody (Catalog # MAB10083). The ND₅₀ is typically 0.3-1.8 µg/mL.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: B7-H2

B7-H2, also called B7RP-1 and ICOSL, is a 60 kDa member of the B7 family of immune costimulatory proteins. It is expressed on resting B cells, dendritic cells, monocytes, alveolar epithelial cells, and placental trophoblasts. B7-H2 interactions with ICOS promote lymphocyte differentiation and activation. Within the ECD, mouse B7-H2 shares 49% and 70% aa sequence identity with human and rat B7-H2, respectively.

Long Name

B7 Homolog 2

Entrez Gene IDs

23308 (Human); 50723 (Mouse); 499415 (Rat); 102132485 (Cynomolgus Monkey)

Alternate Names

B7H2; B7-H2; B7H2B7 homolog 2; B7-H2B7-related protein 1; B7RP-1; B7RP1B7-like protein Gl50; B7RP-1LICOS; CD275 antigen; CD275; GL50; GL50transmembrane protein B7-H2 ICOS ligand; ICOSL; ICOS-L; ICOSLG; inducible T-cell co-stimulator ligand; KIAA0653ICOS ligand