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Mouse BACH1 Antibody

Catalog #: AF5777
5 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF5777
AF5777-SP
Detection of Mouse BACH1 by Western Blot.
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Citations (5)
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Mouse BACH1 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects endogenous mouse BACH1 in Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant mouse BACH1
Ser133-Gly513
Accession # P97302
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Mouse BACH1 antibody by Western Blot. View Larger

Detection of Mouse BACH1 by Western Blot. Western blot shows lysates of NIH-3T3 mouse embryonic fibroblast cell line. PVDF membrane was probed with 1 µg/mL of Mouse BACH1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5777) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for BACH1 at approximately 110 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: BACH1

BACH1 (BTB and CNC homolog 1; also basic leucine zipper transcription factor 1) is a ubiquitously expressed member of the Bach family of transcription factors. Although its predicted MW is 82 kDa, it runs anomalously at 110 kDa in SDS-PAGE. BACH1 forms noncovalent homodimers, and heterodimers with Maf oncoproteins and p53-related proteins. It apparently serves as an architectural component for gene regulatory protein complexes. Mouse BACH1 is 739 amino acids (aa) in length. It contains a protein-interaction BTB domain (aa 24‑127), a DNA-binding motif (aa 565‑580), and a Leu-zipper domain (aa 588‑610). This molecule should not be confused with Bach1/Fancj/Brip1 helicase. Over aa 133‑513, mouse BACH1 shares 75% and 88% aa identity with human and rat BACH1, respectively.

Long Name
BTB and CNC homolog 1
Entrez Gene IDs
571 (Human); 12013 (Mouse); 304127 (Rat)
Alternate Names
BACH1; basic region leucine zipper transcriptional regulator BACH1; BTB and CNC homolog 1; BTB and CNC homology 1, basic leucine zipper transcription factor 1; BTB; HA2303; transcription regulator protein BACH1; transcription regulator protein10BACH-1

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Citations for Mouse BACH1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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  1. Nrf2 controls iron homoeostasis in haemochromatosis and thalassaemia via Bmp6 and hepcidin
    Authors: Pei Jin Lim, Tiago L. Duarte, João Arezes, Daniel Garcia-Santos, Amel Hamdi, Sant-Rayn Pasricha et al.
    Nature Metabolism
  2. Nrf2 Activation Promotes Lung Cancer Metastasis by Inhibiting the Degradation of Bach1
    Authors: Luca Lignitto, Sarah E. LeBoeuf, Harrison Homer, Shaowen Jiang, Manor Askenazi, Triantafyllia R. Karakousi et al.
    Cell
  3. PGRMC2 is an intracellular haem chaperone critical for adipocyte function
    Authors: A Galmozzi, BP Kok, AS Kim, JR Montenegro, JY Lee, R Spreafico, S Mosure, V Albert, R Cintron-Co, C Godio, WR Webb, B Conti, LA Solt, D Kojetin, CG Parker, JJ Peluso, JK Pru, G Siuzdak, BF Cravatt, E Saez
    Nature, 2019-11-20;0(0):.
    Species: Mouse
    Sample Types: Tissue Lysates
    Applications: Western Blot
  4. Specific sequences of infectious challenge lead to secondary hemophagocytic lymphohistiocytosis-like disease in mice
    Authors: A Wang, SD Pope, JS Weinstein, S Yu, C Zhang, CJ Booth, R Medzhitov
    Proc. Natl. Acad. Sci. U.S.A., 2019-01-23;0(0):.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  5. Inflammation induces stress erythropoiesis through heme-dependent activation of SPI-C.
    Authors: Bennett L F, Liao C et al.
    Sci Signal

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