Mouse Bad N-Terminus Antibody

Name: Mouse Bad N-Terminus Antibody
Brand: R&D Systems
SKU: MAB850
Rating: 5 (1 reviews)

Catalog #: MAB850
1 Citation 1 Review Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB850
MAB850-SP

Detection of Mouse Bad N-Terminus by Western Blot.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse Bad N-Terminus Antibody Summary

Species Reactivity

Mouse

Specificity

Detects endogenous mouse Bad in Western blots. In Western blots, this antibody does not cross-react with human Bad. The antibody also detects a 32 kDa band in Western blots.

Source

Monoclonal Rat IgG_2A Clone # 64103

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant mouse Bad

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Immunocytochemistry

5-25 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Mouse Bad N-Terminus by Western Blot. Western blot shows lysates of CTLL-2 mouse cytotoxic T cell line and L-929 mouse fibroblast cell line. PVDF membrane was probed with 1 µg/mL of Rat Anti-Mouse Bad N-Terminus Monoclonal Antibody (Catalog # MAB850) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Bad N-Terminus at approximately 22 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

Immunocytochemistry

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Bad in NIH‑3T3 Mouse Cell Line. Bad was detected in immersion fixed NIH-3T3 mouse embryonic fibroblast cell line using Rat Anti-Mouse Bad N-Terminus Monoclonal Antibody (Catalog # MAB850) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Bad

Bcl-2 antagonist of cell death (Bad) is an 18 kDa cytoplasmic protein in the Bcl-2 family. It functions as a pro-apoptotic molecule by dimerizing with and inhibiting the anti-apoptotic proteins Bcl-2 and Bcl-xL. Prosurvival signals trigger the phosphorylation of Bad on Ser75, Ser99, and Ser115, disrupting its interaction with Bcl-2 and Bcl-xL and resulting in protection from apoptosis. Phosphorylation of Ser75 and Ser99 is also required for the ability of Bad to induce cell cycle arrrest in G1. Human Bad shares 75 % aa sequence identity with mouse and rat Bad.

Long Name

Bcl-xL/Bcl-2 Associated Death Promoter

Entrez Gene IDs

572 (Human); 12015 (Mouse); 64639 (Rat)

Alternate Names

Bad; BBC2; BBC6; bcl2 antagonist of cell death; BCL2-antagonist of cell death protein; BCL2-associated agonist of cell death; Bcl-2-binding component 6; BCL2-binding component 6; BCL2-binding protein; BCL2L8; Bcl2-L-8; BCL2L8bcl2-L-8; Bcl-2-like protein 8; BCL-X/BCL-2 binding protein; Bcl-XL/Bcl-2-associated death promoter

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citation for Mouse Bad N-Terminus Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

Caspase-mediated Cleavage of Insulin Receptor Substrate.
Authors: Green KA, Naylor MJ, Lowe ET, Wang P, Marshman E, Streuli CH
J. Biol. Chem., 2004-04-05;279(24):25149-56.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot