Home / BAFF/BLyS/TNFSF13B / Mouse BAFF/BLyS/TNFSF13B Antibody

Mouse BAFF/BLyS/TNFSF13B Antibody

Catalog #: MAB1357
11 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB1357-SP
MAB1357-500
MAB1357-100
Detection of BAFF/BLyS/TNFSF13B in Mouse Splenocytes by Flow Cytometry
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Citations (11)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse BAFF/BLyS/TNFSF13B Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse BAFF in direct ELISAs and Western blots. In Western blots, this antibody does not cross-react with recombinant mouse (rm) 4-1BB Ligand, rmEDA, rmFas Ligand, rmOX40 Ligand, rmTNF-alpha, rmTRANCE, rmTWEAK, recombinant human (rh) APRIL, rhBAFF, rhEDA-A2, rhGITR Ligand, rhLIGHT, rhLymphotoxin alpha 1/ beta 2, rhLymphotoxin alpha 2/ beta 1, rhTNF-alpha, rhTRAIL, rhVEGI, recombinant canine, cotton rat, equine, feline, porcine, or rat TNF-alpha.
Source
Monoclonal Rat IgG2A Clone # 121808
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse BAFF
Ala127-Leu309
Accession # Q9WU72
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Mouse BAFF/BLyS/TNFSF13B (Catalog # 2106-BF)
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Intracellular Staining by Flow Cytometry
2.5 µg/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry View Larger

Detection of BAFF/BLyS/TNFSF13B in Mouse Splenocytes by Flow Cytometry Mouse Splenocytes were stained with Rat Anti-Mouse BAFF/BLyS/TNFSF13B Monoclonal Antibody (Catalog # MAB1357, filled histogram) or isotype control antibody (Catalog # MAB006, open histogram) followed by Phycoerythrin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0105B). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: BAFF/BLyS/TNFSF13B

BAFF (also known as TALL-1, BLyS, THANK) is a type II transmembrane glycoprotein belonging to the TNF superfamily and has been designated as TNF superfamily member 13B (TNFSF13B). Mouse BAFF is a 309 aa protein consisting of a 248 aa extracellular domain, a 21 aa transmembrane region and a 45 aa cytoplasmic tail (1, 2). BAFF has the typical structural characteristics of the TNF superfamily ligands. It is a homotrimeric protein having the structurally conserved motif known as TNF homology domain (3, 4). A higher ordered structure composed of a cluster of trimeric units resembling the structure of a viral capsid has also been reported (4). Mouse BAFF may be shed from the cell surface by proteolytic cleavage between R126 and Ala 127 to yield a soluble form of the protein detectable in serum (1, 5). Within the TNF superfamily BAFF shares the highest homology (48%) with APRIL (1). BAFF shares with APRIL the ability to bind to BCMA and TACI and also binds specifically to BAFF receptor(BAFF R, also known as BR3 or TNFSFR13C), which is the principal BAFF receptor (6 - 8). All three receptors are type III transmembrane proteins that are expressed in B cells. BAFF and APRIL can form active heteromers that bind TACI (9). BAFF is expressed in peripheral blood mononuclear cells, in spleen and lymph nodes. Its expression in resting monocytes is upregulated by IFN-alpha, IFN-beta, LPS and IL-10. BAFF provides critical survival signals to a subset of B cells with intermediate maturation status (T2 B cells) during the immune response (10). BAFF also plays an important role in the development of lymphoid tissue and enhances the survival of activated memory B cells (7, 11). Human and mouse BAFF share 86% aa sequence identity (1).

References
  1. Schneider, P. et al. (1999) J. Exp. Med. 189:1747.
  2. Mukhopadhyay, A. et al. (1999) J. Biol. Chem. 274:15978.
  3. Karpusas, M. et al. (2002) J. Mol. Biol. 315:1145. 
  4. Liu, Y. et al. (2002) Cell 108:383. 
  5. Cheema, G.S. et al. (2001) Arthr. Rheum. 44:1313. 
  6. Marsters, S.A. et al. (2000) Curr. Biol. 10:785.
  7. Thompson, J.S. et al. (2001) Science 293:2108.
  8. Ng, L. G. et al. (2004) J. Immunol. 173:807.
  9. Roschke, V. et al. (2002) J. Immunol. 169:4314.
  10. Batten, M. et al. (2000) J. Exp. Med. 192:1453.
  11. Avery, D.T. et al. (2003) J. Clin. Invest. 112:286.
Long Name
B cell Activating Factor
Entrez Gene IDs
10673 (Human); 24099 (Mouse); 498666 (Rat); 102123214 (Cynomolgus Monkey)
Alternate Names
ApoL related ligand TALL-1; B lymphocyte stimulator; BAFF; BAFFB-cell activating factor; B-cell-activating factor; BLyS; BLYSB-lymphocyte stimulator; CD257 antigen; CD257; Dendritic cell-derived TNF-like molecule; DTL; TALL1; TALL-1delta BAFF; TALL1Delta4 BAFF; THANK; TNF- and APOL-related leukocyte expressed ligand 1; TNF and ApoL-related leukocyte expressed ligand 1; TNF homolog that activates apoptosis; TNFSF13B; TNFSF20; tumor necrosis factor (ligand) superfamily, member 13b; tumor necrosis factor (ligand) superfamily, member 20; tumor necrosis factor ligand superfamily member 13B; tumor necrosis factor superfamily, member 13b; tumor necrosis factor-like protein ZTNF4; ZTNF4

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Citations for Mouse BAFF/BLyS/TNFSF13B Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

11 Citations: Showing 1 - 10
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  1. BAFF inhibition attenuates fibrosis in scleroderma by modulating the regulatory and effector B cell balance
    Authors: T Matsushita, T Kobayashi, K Mizumaki, M Kano, T Sawada, M Tennichi, A Okamura, Y Hamaguchi, Y Iwakura, M Hasegawa, M Fujimoto, K Takehara
    Sci Adv, 2018-07-11;4(7):eaas9944.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. Regulation of type 1 diabetes development and B-cell activation in nonobese diabetic mice by early life exposure to a diabetogenic environment
    Authors: A De Riva, M Wållberg, F Ronchi, R Coulson, A Sage, L Thorne, I Goodfellow, KD McCoy, M Azuma, A Cooke, R Busch
    PLoS ONE, 2017-08-03;12(8):e0181964.
    Species: Mouse
    Sample Types: Serum
    Applications: ELISA Development (Capture)
  3. B cell homeostasis and follicle confines are governed by fibroblastic reticular cells.
    Authors: Cremasco, Viviana, Woodruff, Matthew, Onder, Lucas, Cupovic, Jovana, Nieves-Bonilla, Janice M, Schildberg, Frank A, Chang, Jonathan, Cremasco, Floriana, Harvey, Christop, Wucherpfennig, Kai, Ludewig, Burkhard, Carroll, Michael, Turley, Shannon
    Nat Immunol, 2014-08-24;15(10):973-81.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  4. Interferon regulatory factor-5 deficiency ameliorates disease severity in the MRL/lpr mouse model of lupus in the absence of a mutation in DOCK2.
    Authors: Yasuda K, Watkins A, Kochar G, Wilson G, Laskow B, Richez C, Bonegio R, Rifkin I
    PLoS ONE, 2014-07-30;9(7):e103478.
    Species: Mouse
    Sample Types: Serum
    Applications: ELISA Development (Capture)
  5. Nitric oxide regulates BAFF expression and T cell-independent antibody responses.
    Authors: Giordano D, Draves K, Li C, Hohl T, Clark E
    J Immunol, 2014-06-20;193(3):1110-20.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  6. HIV-1 adenoviral vector vaccines expressing multi-trimeric BAFF and 4-1BBL enhance T cell mediated anti-viral immunity.
    Authors: Kanagavelu, Saravana, Termini, James M, Gupta, Sachin, Raffa, Francesc, Fuller, Katherin, Rivas, Yaelis, Philip, Sakhi, Kornbluth, Richard, Stone, Geoffrey
    PLoS ONE, 2014-02-28;9(2):e90100.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  7. Local BLyS production by T follicular cells mediates retention of high affinity B cells during affinity maturation.
    Authors: Goenka, Radhika, Matthews, Andrew H, Zhang, Bochao, O'Neill, Patrick, Scholz, Jean L, Migone, Thi-Sau, Leonard, Warren J, Stohl, William, Hershberg, Uri, Cancro, Michael
    J Exp Med, 2013-12-23;211(1):45-56.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  8. Myeloid cells, BAFF, and IFN-gamma establish an inflammatory loop that exacerbates autoimmunity in Lyn-deficient mice.
    Authors: Scapini P, Hu Y, Chu CL
    J. Exp. Med., 2010-07-12;207(8):1757-73.
    Species: Mouse
    Sample Types: Serum
    Applications: ELISA Development
  9. B-cell activating factor (BAFF) plays a role in the mechanism of action of a tolerogenic peptide that ameliorates lupus.
    Authors: Parameswaran R, Ben David H, Sharabi A, Zinger H, Mozes E
    Clin. Immunol., 2009-02-01;131(2):223-32.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  10. Direct B cell stimulation by dendritic cells in a mouse model of lupus.
    Authors: Wan S, Zhou Z, Duan B, Morel L
    Arthritis Rheum., 2008-06-01;8(0):1741-50.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  11. Pharmacological potentiation of the efferent vagus nerve attenuates blood pressure and renal injury in a murine model of systemic lupus erythematosus
    Authors: Grace S. Pham, Lei A. Wang, Keisa W. Mathis
    American Journal of Physiology-Regulatory, Integrative and Comparative Physiology

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