Mouse BTN1A1/Butyrophilin Antibody Summary
Ala27-Trp247
Accession # Q62556
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Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Mouse BTN1A1/Butyrophilin by Western Blot. Western blot shows lysates of mouse lactating mammary tissue. PVDF membrane was probed with 0.25 µg/mL of Sheep Anti-Mouse BTN1A1/Butyrophilin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4765) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for BTN1A1/Butyrophilin at approximately 66 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: BTN1A1/Butyrophilin
Butyrophilin (Greek for “being like butter”; also Btn1a1) is a 66 kDa member of the BTN/MOG family of proteins. It is expressed on secretory mammary epithelium and associates with xanthine dehydrogenase. This is necessary for milk-lipid droplet secretion. Mature mouse Butyrophilin is 498 amino acids (aa) in length. It is a type I transmembrane glycoprotein that contains a 221 aa extracellular domain (ECD) (aa 27‑247) and a 256 aa cytoplasmic region. The ECD contains two V-type Ig‑like domains (aa 29‑141 and aa 149‑235). There are two potential splice variants. One shows a deletion of aa 145‑238, while a second shows a 36 aa substitution for aa 144‑524. Over aa 27‑247, mouse Butyrophilin shares 85% and 67% aa identity with rat and human Butyrophilin, respectively.
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Day 6 lactating mouse mammary gland tissue. Antigen retrieval with Tris EDTA (pH 9). Blocked with 50% serum, permeabolized with 0.3% TritonX-100. Used 1:50 dilution of anti-Btn1a1, @RT 1 hour. Detection was with anti-sheep HRP (1:200; 1hour RT) then anti-HRP Alexa Fluor 488 (1:50; 1hour RT). Nuclei stained with DAPI.
