Mouse c-Rel Antibody

Catalog # Availability Size / Price Qty
MAB2699
MAB2699-SP
Detection of Mouse c-Rel by Western Blot.
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Product Details
Citations (2)
FAQs
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Mouse c-Rel Antibody Summary

Species Reactivity
Mouse
Specificity
Detects endogenous mouse c-Rel in Western blots.
Source
Monoclonal Rat IgG2A Clone # 290512
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant mouse c-Rel
Met1-Ile588
Accession # P15307
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
0.5 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Mouse c-Rel antibody by Western Blot. View Larger

Detection of Mouse c-Rel by Western Blot. Western blot shows lysates of DA3 mouse myeloma cell line, A20 mouse B cell lymphoma cell line, and CH-1 mouse B cell lymphoma cell line. PVDF membrane was probed with 0.5 µg/mL of Mouse c-Rel Monoclonal Antibody (Catalog # MAB2699) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for c-Rel at approximately 80 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: c-Rel

c-Rel is a member of the NF kappa B/Rel family of transcription factors. NF kappa B/Rel dimers containing c-Rel regulate expression of genes involved in lymphoid cell development, proliferation, and survival.

Long Name
Cellular Repressor of E1A-stimulated Genes
Entrez Gene IDs
5966 (Human); 19696 (Mouse)
Alternate Names
C-Rel proto-oncogene protein; cRel; c-Rel; I-Rel; oncogene REL, avian reticuloendotheliosis; proto-oncogene c-Rel; REL; v-rel avian reticuloendotheliosis viral oncogene homolog; v-rel reticuloendotheliosis viral oncogene homolog (avian)

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Citations for Mouse c-Rel Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Human Papillomavirus 16 E7 Promotes EGFR/PI3K/AKT1/NRF2 Signaling Pathway Contributing to PIR/NF-kappa B Activation in Oral Cancer Cells
    Authors: Diego Carrillo-Beltrán, Juan P. Muñoz, Nahir Guerrero-Vásquez, Rancés Blanco, Oscar León, Vanesca de Souza Lino et al.
    Cancers (Basel)
  2. IkappaBNS regulates murine Th17 differentiation during gut inflammation and infection.
    Authors: Annemann M, Wang Z, Plaza-Sirvent C, Glauben R, Schuster M, Ewald Sander F, Mamareli P, Kuhl A, Siegmund B, Lochner M, Schmitz I
    J Immunol, 2015-02-18;194(6):2888-98.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot

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