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Mouse C5L2/GPR77 Antibody

Catalog #: MAB4729
2 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB4729
MAB4729-SP
Detection of C5L2/GPR77 in RAW 264.7 Mouse Cell Line by Flow Cytometry.
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Citations (2)
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Mouse C5L2/GPR77 Antibody Summary

Species Reactivity
Mouse
Specificity
This antibody stains mouse C5L2 transfectants but not irrelevant transfectants.
Source
Monoclonal Rat IgG2B Clone # 468705
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Chinese hamster ovary cell line transfected with mouse C5L2
Met1-Val344
Accession # Q8BW93
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Intracellular Staining by Flow Cytometry
0.25 µg/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Intracellular Staining by Flow Cytometry Detection of C5L2/GPR77 antibody in RAW 264.7 Mouse Cell Line antibody by Flow Cytometry. View Larger

Detection of C5L2/GPR77 in RAW 264.7 Mouse Cell Line by Flow Cytometry. RAW 264.7 mouse monocyte/macrophage cell line was stained with Rat Anti-Mouse C5L2/GPR77 Monoclonal Antibody (Catalog # MAB4729, filled histogram) or isotype control antibody (Catalog # MAB0061, open histogram), followed by Phycoerythrin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0105B). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: C5L2/GPR77

C5L2, also known as GPR77, is a 45 kDa 7TM receptor that binds the complement proteins C3a, C4a, and C5a as well as the acylation stimulating peptide (ASP). C5L2 is expressed on various hematopoietic cells where it promotes complement-mediated inflammation. ASP binding to C5L2 on adipocytes triggers triglyceride synthesis and glucose uptake and inhibits lipolysis. Human C5L2 shares 60% amino acid sequence identity with mouse and rat C5L2.

Long Name
C5a Anaphylatoxin Chemotactic Receptor C5L2
Entrez Gene IDs
27202 (Human); 319430 (Mouse); 445269 (Rat)
Alternate Names
C5a anaphylatoxin chemotactic receptor C5L2; C5L2; C5L2G-protein coupled receptor 77; G protein-coupled receptor 77; G protein-coupled receptor C5L2; GPF77; GPR77

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Citations for Mouse C5L2/GPR77 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. T Cell Expression of C5a Receptor 2 Augments Murine Regulatory T Cell (T) Generation and T-Dependent Cardiac Allograft Survival
    Authors: D A Verghese, M Demir, N Chun, M Fribourg, P Cravedi, I Llaudo, TM Woodruff, P Yadav, SA Lira, ME Medof, PS Heeger
    J. Immunol., 2018-02-07;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. Monitoring C5aR2 Expression Using a Floxed tdTomato-C5aR2 Knock-In Mouse
    Authors: CM Karsten, AV Wiese, F Mey, J Figge, TM Woodruff, T Reuter, O Scurtu, A Kordowski, LN Almeida, D Briukhovet, KM Quell, J Sun, F Ender, I Schmudde, T Vollbrandt, Y Laumonnier, J Köhl
    J. Immunol., 2017-09-01;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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