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Mouse Carboxylesterase 1/CES1 Antibody

Catalog #: AF7929 1 Review Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF7929
AF7929-SP
Detection of Mouse Carboxylesterase 1/ CES1 by Western Blot.
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Mouse Carboxylesterase 1/CES1 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse Carboxylesterase 1/CES1 in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant mouse CES3 and recombinant human CES1 is observed.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Carboxylesterase 1/CES1
His19-Leu565
Accession # Q8VCC2
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Mouse Carboxylesterase 1/ CES1 antibody by Western Blot. View Larger

Detection of Mouse Carboxylesterase 1/ CES1 by Western Blot. Western blot shows lysates of mouse liver. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Mouse Carboxylesterase 1/CES1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7929) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Carboxylesterase 1/CES1 at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Carboxylesterase 1/CES1

Carboxylesterase 1/CES1 (also known as Ses-1 and Liver carboxylesterase 1) is a 60-63 kDa glycoprotein, member of a type-B carboxylesterase/lipase family localized to microsomal membrane and  lumen of endoplasmic reticulum. CES1 exists as a homotrimer and homohexamer and is involved in the detoxification of xenobiotics and in the activation of ester and amide prodrugs. CES1 is expressed mostly in liver, and with lower levels found in the lung. Over amino acids (aa) 19‑565, mouse CES1 shares 75% aa identity with human CES1.   

Entrez Gene IDs
1066 (Human); 12623 (Mouse); 29225 (Rat)
Alternate Names
ACAT; acyl coenzyme A:cholesterol acyltransferase; Acyl-coenzyme A:cholesterol acyltransferase; Brain carboxylesterase hBr1; carboxylesterase 1 (monocyte/macrophage serine esterase 1); Carboxylesterase 1; carboxylesterase 2 (liver); CEH; CES1; Ces-1; CES1A2; CES2EC 3.1.1.1; cholesteryl ester hydrolase; Cocaine carboxylesterase; EC 3.1.1; Egasyn; HMSE1; HMSECES1A1; human monocyte/macrophage serine esterase 1; liver carboxylesterase 1; MGC117365; Monocyte/macrophage serine esterase; PCE-1; REH; Retinyl ester hydrolase; Serine esterase 1; SES1; Ses-1; SES1TGH; TGH; Triacylglycerol hydrolase

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Mouse Carboxylesterase 1/CES1 Antibody
By Hua Jiang on 06/01/2016
Application: WB Sample Tested: Liver tissue Species: Human

Primary ab dilution 1:2000
40 ug protein per lane

Western Blot Mouse Carboxylesterase 1/CES1 Antibody AF7929